PXD056161

PXD056161 is an original dataset announced via ProteomeXchange.

Title	Metabolomic and Proteomic Analysis of Breast Cancer Exosomes Reveals Alterations in Purine and Carnitine Metabolism
Description	A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased purine synthesis intermediates and increased carnitine synthesis metabolites in MDA-MB-231-derived exosomes, with pathway enrichment indicating activation of de novo purine pathways and upregulating of carnitine metabolism. In addition, nanoLC-MS-based proteomics revealed differential expression of ecto-5’-nucleotidase (NT5E) and mitochondrial aldehyde dehydrogenase (ALDH9A1) demonstrated metabolic alterations in related enzymatic steps. This study demonstrates the application of nano sheath-flow CE-MS for comprehensive and quantitative exosome metabolomics, uncovering metabolic reprogramming in purine and carnitine pathways between normal and cancerous breast cell lines and providing insight into exosome-mediated signaling of breast cancer metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-03-25
AnnouncementXML	Submission_2025-03-25_09:27:46.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abdullah Khraibah
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	alkylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 02:52:23	ID requested
⏵ 1	2025-03-25 09:27:47	announced

10.1021/ACS.JPROTEOME.4C00795;

submitter keyword: Metabolomics, Breast Cancer,Capillary Electrophoresis-Mass Spectrometry, Proteomics

Maxim Berezovski
contact affiliation	Department of Chemistry and Biomolecular Sciences, University of Ottawa, Canada
contact email	Maxim.Berezovski@uOttawa.ca
lab head
Abdullah Khraibah
contact affiliation	University of Ottawa
contact email	akhra046@uottawa.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056161
     1. Label: PRIDE project
     2. Name: Metabolomic and Proteomic Analysis of Breast Cancer Exosomes Reveals Alterations in Purine and Carnitine Metabolism