⮝ Full datasets listing

PXD056161

PXD056161 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMetabolomic and Proteomic Analysis of Breast Cancer Exosomes Reveals Alterations in Purine and Carnitine Metabolism
DescriptionA nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased purine synthesis intermediates and increased carnitine synthesis metabolites in MDA-MB-231-derived exosomes, with pathway enrichment indicating activation of de novo purine pathways and upregulating of carnitine metabolism. In addition, nanoLC-MS-based proteomics revealed differential expression of ecto-5’-nucleotidase (NT5E) and mitochondrial aldehyde dehydrogenase (ALDH9A1) demonstrated metabolic alterations in related enzymatic steps. This study demonstrates the application of nano sheath-flow CE-MS for comprehensive and quantitative exosome metabolomics, uncovering metabolic reprogramming in purine and carnitine pathways between normal and cancerous breast cell lines and providing insight into exosome-mediated signaling of breast cancer metabolism.
HostingRepositoryPRIDE
AnnounceDate2025-03-25
AnnouncementXMLSubmission_2025-03-25_09:27:46.631.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAbdullah Khraibah
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListalkylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-24 02:52:23ID requested
12025-03-25 09:27:47announced
Publication List
10.1021/ACS.JPROTEOME.4C00795;
Keyword List
submitter keyword: Metabolomics, Breast Cancer,Capillary Electrophoresis-Mass Spectrometry, Proteomics
Contact List
Maxim Berezovski
contact affiliationDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Canada
contact emailMaxim.Berezovski@uOttawa.ca
lab head
Abdullah Khraibah
contact affiliationUniversity of Ottawa
contact emailakhra046@uottawa.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD056161
PRIDE project URI
Repository Record List
[ + ]