PXD056161 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metabolomic and Proteomic Analysis of Breast Cancer Exosomes Reveals Alterations in Purine and Carnitine Metabolism |
Description | A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased purine synthesis intermediates and increased carnitine synthesis metabolites in MDA-MB-231-derived exosomes, with pathway enrichment indicating activation of de novo purine pathways and upregulating of carnitine metabolism. In addition, nanoLC-MS-based proteomics revealed differential expression of ecto-5’-nucleotidase (NT5E) and mitochondrial aldehyde dehydrogenase (ALDH9A1) demonstrated metabolic alterations in related enzymatic steps. This study demonstrates the application of nano sheath-flow CE-MS for comprehensive and quantitative exosome metabolomics, uncovering metabolic reprogramming in purine and carnitine pathways between normal and cancerous breast cell lines and providing insight into exosome-mediated signaling of breast cancer metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-25 |
AnnouncementXML | Submission_2025-03-25_09:27:46.631.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Abdullah Khraibah |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | alkylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-24 02:52:23 | ID requested | |
⏵ 1 | 2025-03-25 09:27:47 | announced | |
Publication List
Keyword List
submitter keyword: Metabolomics, Breast Cancer,Capillary Electrophoresis-Mass Spectrometry, Proteomics |
Contact List
Maxim Berezovski |
contact affiliation | Department of Chemistry and Biomolecular Sciences, University of Ottawa, Canada |
contact email | Maxim.Berezovski@uOttawa.ca |
lab head | |
Abdullah Khraibah |
contact affiliation | University of Ottawa |
contact email | akhra046@uottawa.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056161
- Label: PRIDE project
- Name: Metabolomic and Proteomic Analysis of Breast Cancer Exosomes Reveals Alterations in Purine and Carnitine Metabolism