PXD056157

PXD056157 is an original dataset announced via ProteomeXchange.

Title	The butyrate-producing Gram-positive human gut bacterium, Hoskinsella mucinilytica, selectively targets host mucin N-acetylhexosamines
Description	The personalized communities of microorganisms (microbiota) that inhabit the distal guts of humans have evolved to process a variety of complex carbohydrates. Many gut bacteria depolymerize and ferment dietary fiber polysaccharides, mutualistically providing short-chain fatty-acids to their host. Some human gut bacteria have evolved to utilize components of host mucin glycoproteins—the major component of protective mucus—and recent studies have implicated specific mucin-degrading commensal bacteria in the development of intestinal inflammation. Thus, identifying new gut bacteria that possess the ability to degrade host mucins and determining how these organisms might cooperate or compete for mucin components during health and disease is an important goal. Here, we used culture on solid medium containing gastric mucin as the main carbon source to isolate a novel bacterium that is largely restricted to using the N-acetylglucosamine and N-acetylgalactosamine sugars found in mucin O-glycans. This butyrate-producing bacterium accesses these sugars from both polymeric gastric mucin and chemically released oligosaccharides and has a genome with correspondingly restricted carbohydrate-active enzyme content. Surprisingly, strains related to this isolate appear to be rare in the now numerous sequence-based microbiota surveys with only 32/3781 (0.85%) human subjects containing this bacterium with an overall relative abundance ranging from 0.004-0.057%. This combination of low prevalence and abundance, suggests that this species could occupy an unknown niche in which access to mucin is important but otherwise render it difficult to detect in mostly feces-based microbiota surveys.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:09:37.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicholas Pudlo
SpeciesList	scientific name: Erysipelotrichaceae bacterium; NCBI TaxID: NEWT:2049044;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 02:42:29	ID requested
⏵ 1	2026-04-26 16:09:38	announced

10.1016/j.jbc.2026.111371;

Pudlo NA, Pereira GV, Schaus SR, Yang Q, Fuentes JJ, Jin C, Hein R, Zhang L, Terrapon N, Lyssiotis CA, Schmidt TM, Hansson GC, Luis AS, Martens EC, The butyrate-producing Gram-positive human gut bacterium, Hoskinsella mucinilytica, selectively targets host mucin N-acetylhexosamines. J Biol Chem, 302(5):111371(2026) [pubmed]

submitter keyword: microbiota, mucin, bacteria

Eric C Martens
contact affiliation	Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI USA.
contact email	emartens@umich.edu
lab head
Nicholas Pudlo
contact affiliation	University of Michigan
contact email	npudlo@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD056157

PRIDE project URI

• PRIDE
  1. PXD056157
     1. Label: PRIDE project
     2. Name: The butyrate-producing Gram-positive human gut bacterium, Hoskinsella mucinilytica, selectively targets host mucin N-acetylhexosamines