PXD056153

PXD056153 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	mouse embryonic stem cells heterochromatin-associated protein and Nat10 IP-MS
Description	The sonication-resistant heterochromatin (srHC) fragments of mESCs were isolated by sucrose gradient ultracentrifugation, and the heterochromatin-associated proteins were enriched by Co-IP combined with mass spectrometry (Co-IP/MS) using anti-H3K9me3, IgG was used as a negative control. The Nat10 interacted proteins in mESCs were isolated by 3×Flag-Nat10 fusion protein overexpression and performed IP-MS using anti-Flag, IgG antibody was used as a negative control.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-22_16:06:58.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoxing Yin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 01:53:35	ID requested
⏵ 1	2025-06-22 16:06:59	announced

Publication List

Feng G, Yin G, Liu Y, Liu C, Li J, Lu J, Yu Y, Jin Z, Wu Y, Yue Y, Gao X, Wang J, Lu F, Liu L, Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation. Nucleic Acids Res, 53(11):(2025) [pubmed]
10.1093/nar/gkaf504;

Feng G, Yin G, Liu Y, Liu C, Li J, Lu J, Yu Y, Jin Z, Wu Y, Yue Y, Gao X, Wang J, Lu F, Liu L, Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation. Nucleic Acids Res, 53(11):(2025) [pubmed]

10.1093/nar/gkaf504;

Keyword List

submitter keyword: Nat10 interacted proteins in mESCs,Heterochromatin-associated proteins in mESCs

submitter keyword: Nat10 interacted proteins in mESCs,Heterochromatin-associated proteins in mESCs

Contact List

Guofeng Feng
contact affiliation	Nankai University
contact email	fengguofeng_121@163.com
lab head
Guoxing Yin
contact affiliation	Nankai University
contact email	1083334205@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD056153
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD056153

PRIDE project URI

Repository Record List

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