PXD056099

PXD056099 is an original dataset announced via ProteomeXchange.

Title	TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing – data-independent acquisition MS
Description	TFIID is an essential basal transcription factor, crucial for RNA polymerase II (pol II) promoter recognition and transcription initiation. The TFIID complex consists of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs) that contain intrinsically disordered regions (IDRs) with currently unknown functions. Here, we show that a conserved IDR drives TAF2 condensation in nuclear speckles, independently of other TFIID subunits. Quantitative mass spectrometry analyses reveal that TAF2 exhibits close proximities to RNA-binding proteins, but that the TAF2 IDR specifically interacts with the splicing factor SRRM2. Modulation of TAF2 protein levels elicits alternative splicing (AS) events in RNAs coding for proteins involved in transcription and transmembrane transport, some of which overlap with SRRM2-regulated AS events. This study identifies an IDR of the basal transcription machinery as a molecular guide for protein partitioning into nuclear compartments, controlling protein complex composition and pre-mRNA splicing.
HostingRepository	PRIDE
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-04_04:04:58.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 09:16:18	ID requested
⏵ 1	2025-03-04 04:04:58	announced

Dataset with its publication pending

submitter keyword: TAF2,Transcription, intrinsically disordered regions, nuclear speckles, biomolecular condensates, TFIID, splicing, SRRM2, nuclear bodies

Pitter Huesgen
contact affiliation	Faculty of Biology, University of Freiburg, Freiburg, Germany
contact email	pitter.huesgen@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	University of Freiburg
contact email	f.drepper@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056099
     1. Label: PRIDE project
     2. Name: TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing – data-independent acquisition MS