PXD056083

PXD056083 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of specific mucosal regions of Salmonella-infected mouse cecum
Description	The continuously remodeled extracellular matrix (ECM) plays a pivotal role in gastrointestinal health and disease, yet its precise functions remain elusive. In this project, we employed laser capture microdissection of cecal mucosa combined with low-input proteomics to investigate ECM remodeling during Salmonella-driven inflammation. To complement this, we probed how fibronectin fiber tension is altered using a mechanosensitive peptide probe. While fibronectin fibers in healthy intestinal tissue are typically stretched, fibronectin fiber relaxation occurred exclusively during late-stage infection at 72 hours and was localized to already existing clusters of infiltrated neutrophils in the cecal mucosa of Salmonella-infected mice.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:49:40.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronja Rappold
SpeciesList	scientific name: Salmonella enterica; NCBI TaxID: NCBITaxon:28901; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 04:09:03	ID requested
⏵ 1	2025-05-07 02:49:41	announced

10.1038/s44341-024-00006-y;

Rappold R, Kalogeropoulos K, La Regina G, Auf dem Keller U, Slack E, Vogel V, Relaxation of mucosal fibronectin fibers in late gut inflammation following neutrophil infiltration in mice. NPJ Biol Phys Mech, 2(1):4(2025) [pubmed]

submitter keyword: mouse, salmonella, fibronectin, laser capture microdissection

Viola Vogel
contact affiliation	D-HEST, ETH Zurich
contact email	viola.vogel@hest.ethz.ch
lab head
Ronja Rappold
contact affiliation	ETH Zurich
contact email	rrappold@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056083

PRIDE project URI

• PRIDE
  1. PXD056083
     1. Label: PRIDE project
     2. Name: Proteomics analysis of specific mucosal regions of Salmonella-infected mouse cecum