PXD056082 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CoIP-LC-MS/MS of Rad50 in KO Vs. WT L3 CNS |
| Description | Cell types are fundamental units of metazoans, however their definition remains a long-standing challenge. We here use high throughput assays allowing for unprecedented resolution and systematic analyses. The comparison of the transcriptional landscapes of related and unrelated Drosophila differentiated cell types at larval and embryonic stages allowed us to identify features that are stable and cell-specific, revealing a unique signature for each cell type. Unexpectedly, all cell types share a stage-specific signature that is even stronger than the cell-specific one. Typically, embryonic hemocytes resemble more embryonic neurons than larval hemocytes. The stage-specific signature is associated to changes in chromatin accessibility between the two analyzed stages. We identified Rad50 as a chromatin boundary associated protein crucial for the temporal regulation of gene expression and chromatin organization, revealing a function of Rad50 beyond DNA repair. The identification of cell-specific and stage-specific signatures as equally important highlights another dimension of cell identity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-26_16:43:18.830.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD056082 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Johana Chicher |
| SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-09-23 03:13:53 | ID requested | |
| ⏵ 1 | 2026-04-26 16:43:19 | announced | |
Publication List
| 10.1093/nar/gkag294; |
| Boutet T, Sakr R, Marzullo M, Goyal M, Cattenoz PB, Ciapponi L, Mukherjee T, Giangrande A, Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in Drosophila. Nucleic Acids Res, 54(7):(2026) [pubmed] |
| 10.6019/PXD056082; |
Keyword List
| submitter keyword: Chromatin |
| Temporal regulation |
| Rad50 |
| Cell identity |
Contact List
| Angela Giangrande |
|---|
| contact affiliation | Department of Functional Genomics and Cancer, IGBMC, Illkirch, France |
| contact email | angela@igbmc.fr |
| lab head | |
| Johana Chicher |
|---|
| contact affiliation | Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique |
| contact email | j.chicher@ibmc-cnrs.unistra.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD056082 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056082
- Label: PRIDE project
- Name: CoIP-LC-MS/MS of Rad50 in KO Vs. WT L3 CNS
