PXD056080

PXD056080 is an original dataset announced via ProteomeXchange.

Title	Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to Interferon induction
Description	The RNA editing enzyme ADAR1, is essential for correct functioning of innate immune responses. The ADAR1p110 isoform is mainly nuclear and ADAR1p150, which is interferon inducible, is predominately cytoplasmic. Using three different approaches, co-IP with endogenous ADAR1, and Strep-tag co-IP and BioID with individual ADAR1 isoforms, a comprehensive interactome was generated during both homeostasis and the IFN response. Both known and novel interactors and editing regulators were identified. Nuclear proteins were detected as stable interactors with both ADAR1 isoforms. In contrast, BioID identified distinct protein networks for each ADAR1 isoform, with nuclear components observed with ADAR1p110 and components of cytoplasmic cellular condensates with ADAR1p150. RNase A digestion distinguished between distal and proximal interactors, as did a dsRNA-binding mutant of ADAR1 which demonstrated the importance of dsRNA binding for ADAR1 interactions. IFN treatment did not affect the core ADAR1 interactomes but resulted in novel interactions, the majority of which are proximal interactions retained after RNase A treatment. Short treatment with HMW poly(I:C) during the IFN response resulted in dsRNA-binding-dependent changes in the proximal protein network of ADAR1p110 and association of the ADAR1p150 proximal protein network with some components of antiviral stress granules.
HostingRepository	PRIDE
AnnounceDate	2024-12-08
AnnouncementXML	Submission_2024-12-08_02:16:36.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dragana Vukic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 01:29:59	ID requested
⏵ 1	2024-12-08 02:16:36	announced

10.1093/NAR/GKAE1106;

submitter keyword: RNase A, endo-IP, human, IFN response,ADAR, A549 cells

Prof. Mary Anne O´Connell, Ph.D.
contact affiliation	CEITEC, Masaryk University Molecular medicine, RNA and immunity, CEITEC, Masaryk University
contact email	mary.oconnell@ceitec.muni.cz
lab head
Dragana Vukic
contact affiliation	Central European Institute for Technology at Masaryk University
contact email	dragana.vukic@ceitec.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056080
     1. Label: PRIDE project
     2. Name: Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to Interferon induction