PXD056077

PXD056077 is an original dataset announced via ProteomeXchange.

Title	Metal ion-decorated ZIC-cHILIC StageTip Enables Simultaneous Profiling of N-Glycoproteome and Phosphoproteome in TKI resistant NSCLC cells
Description	Cell membrane proteins are densely decorated with surface glycosylation and intracellular phosphorylation whose interplay determines the cell-cell communication and signaling cascades. However, their concomitant characterization remains extreme challenges due to complexity of glycan structures, low abundance of glycopeptides (particularly sialylation), labile, dynamic nature and low detectability of both phosphopeptides and glycopeptides in mass spectrometry. In addition, the interplay and dynamic change between glycosylation and phosphorylation in tyrosine kinase inhibitor (TKI)-resistant non-small cell lung cancer (NSCLC) cells is still unclear. In this study, we introduce a streamlined metal ion-decorated ZIC-cHILIC strategy, featuring a simple pH control, to allow simultaneous enrichment and stepwise separation of intact (sialo-)glycopeptides and phosphopeptides.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_19:10:25.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Ju Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; complex glycosylation; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-22 23:16:51	ID requested
⏵ 1	2025-08-03 19:10:26	announced

10.1016/j.mcpro.2025.100957;

Chen YJ, Chen YL, Chang KH, Cheng HC, Chang CC, Chen YJ, Metal Ion-Enhanced ZIC-cHILIC StageTip for N-Glycoproteomic and Phosphoproteomic Profiling in EGFR-Mutated Lung Cancer Cells. Mol Cell Proteomics, 24(6):100957(2025) [pubmed]

submitter keyword: phosphoproteome, N-glycoproteome,ZIC-cHILIC

Yi-Ju Chen
contact affiliation	Assistant Research Specialist, Institute of Chemistry, Academia Sinica
contact email	tp7249@gate.sinica.edu.tw
lab head
Yi-Ju Chen
contact affiliation	Academia Sinica
contact email	tp7249@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD056077

PRIDE project URI

• PRIDE
  1. PXD056077
     1. Label: PRIDE project
     2. Name: Metal ion-decorated ZIC-cHILIC StageTip Enables Simultaneous Profiling of N-Glycoproteome and Phosphoproteome in TKI resistant NSCLC cells