PXD056057

PXD056057 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The zebrafish Danio rerio sperm proteome by LC-MSMS
Description	Fertilisation is based on the tight interaction between sperm and egg and both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the mechanisms involved during fertilisation and the fusion between sperm and egg. Despite being a commonly used model organism for reproductive research, very little is currently understood about the zebrafish Danio rerio sperm proteome. We performed in depth proteomics analysis with six pooled samples containing ejaculates from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms. This number of proteins is larger than the number of proteins described in sperm proteomes of other species.
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_04:29:41.329.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056057
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-20 06:12:10	ID requested
⏵ 1	2025-01-27 04:29:42	announced

Publication List

10.6019/PXD056057;
10.1002/pmic.202400310;
Cohen-Krais J, Martins C, Bartram J, Crighton Z, de Coriolis JC, Godden A, Marcu D, Robak W, Saalbach G, Immler S, The Zebrafish Sperm Proteome. Proteomics, 25(4):e202400310(2025) [pubmed]

10.6019/PXD056057;

10.1002/pmic.202400310;

Cohen-Krais J, Martins C, Bartram J, Crighton Z, de Coriolis JC, Godden A, Marcu D, Robak W, Saalbach G, Immler S, The Zebrafish Sperm Proteome. Proteomics, 25(4):e202400310(2025) [pubmed]

Keyword List

submitter keyword: fertility, proteomics, Danio rerio,fertilisation, sperm function, sperm-egg interaction

submitter keyword: fertility, proteomics, Danio rerio,fertilisation, sperm function, sperm-egg interaction

Contact List

Simone Immler
contact affiliation	Professor School of Biological Sciences University of East Anglia Norwich UK
contact email	s.immler@uea.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD056057
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD056057

PRIDE project URI

Repository Record List

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