PXD056055

PXD056055 is an original dataset announced via ProteomeXchange.

Title	Pex30-dependent membrane contacts sites maintain ER lipid homeostasis
Description	In eukaryotic cells, communication between organelles and the coordination of their activities depend on membrane contact sites (MCS). How MCS are regulated under the dynamic cellular environment remains poorly understood. Here, we investigate how Pex30, a membrane protein localized to the endoplasmic reticulum (ER), regulates multiple MCS in budding yeast. We show that Pex30 is critical for the integrity of ER MCS with peroxisomes and vacuoles. This requires the Dysferlin domain (DysF) on Pex30 cytosolic tail. This domain binds to phosphatidic acid (PA) both in vitro and in silico, and it is important for normal PA metabolism in vivo. The DysF domain is evolutionarily conserved and may play a general role in PA homeostasis across eukaryotes. We further show that ER-Vacuole MCS requires Pex30 C-terminal Domain of Unknown Function and that its activity is controlled by phosphorylation in response to metabolic cues. These findings provide new insights into the dynamic nature of MCS and their coordination with cellular metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-05-08
AnnouncementXML	Submission_2025-05-08_01:57:48.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pedro Carvalho
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-20 05:56:09	ID requested
⏵ 1	2025-05-08 01:57:48	announced

10.1083/JCB.202409039;

submitter keyword: Endoplasmic reticulum

Lipid droplet

Peroxisome

Pex30

NVJ

Membrane contact site

Dysferlin

DUF4196

Phosphatidic acid

Pedro Carvalho
contact affiliation	Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
contact email	pedro.carvalho@path.ox.ac.uk
lab head
Pedro Carvalho
contact affiliation	University of Oxford
contact email	pedro.carvalho@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056055

PRIDE project URI

• PRIDE
  1. PXD056055
     1. Label: PRIDE project
     2. Name: Pex30-dependent membrane contacts sites maintain ER lipid homeostasis