PXD056047 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PDCD6 modulates LC3-associated phagocytosis-mediated antimicrobial defense by regulating lactate metabolism |
Description | LC3-associated phagocytosis (LAP) is critical in host defense against invading pathogens, but the molecular mechanism for LAP activation is still unclear. Here, we find programmed cell death 6 (PDCD6) as a negative regulator of LAP. PDCD6 deficiency in mice and macrophages induces enhanced bactericidal activity and LAP formation. In parallel, lactate dehydrogenase A (LDHA) activity and lactate production is induced in macrophages challenged with bacteria, Zymosan or Pam3CSK4, while genetic ablation or pharmacological inhibition of LDHA reduces lactate levels and impairs bactericidal activity in vivo and in vitro. Mechanistically, PDCD6 interacts with LDHA to downregulate lactate metabolism, leading to reduced RUBCN lactylation at lysine33 (K33). By contrast, PDCD6-deficiency increases RUBCN lactylation, thereby promotes RUBCN interaction with VPS34, LAP formation, and protective responses. Our results thus suggest a PDCD6-LDHA-lactate-RUBCN axis of innate immunity regulation that may both contribute to protection from infectious diseases and serve as targets for therapeutic development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-18 |
AnnouncementXML | Submission_2024-10-18_02:34:16.407.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sun Lulu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-20 02:00:21 | ID requested | |
⏵ 1 | 2024-10-18 02:34:16 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Lactate, LAP,PDCD6 |
Contact List
Tianliang Li |
contact affiliation | College of Life Sciences, Shandong Normal University |
contact email | li.tianliang@outlook.com |
lab head | |
Sun Lulu |
contact affiliation | Shandong Normal University |
contact email | 1723783573@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056047
- Label: PRIDE project
- Name: PDCD6 modulates LC3-associated phagocytosis-mediated antimicrobial defense by regulating lactate metabolism