PXD056029

PXD056029 is an original dataset announced via ProteomeXchange.

Title	Differential Impact of p97 Inhibitor CB-5083 on Protein Dynamics in Nucleus, Cytoplasm, and Membrane Compartments
Description	Human p97/VCP is a vital AAA ATPase (ATPase associated with diverse cellular activity) plays critical roles in autophagy, endosomal trafficking, and the ubiquitin-proteasome system. Mutations in p97 cause inclusion body myopathy associated with paget’s disease of bone and frontotemporal dementia. P97 is overexpressed in several cancers. The selective active site inhibitor CB-5083 targets p97’s D2 domain and shows potential as an anti-cancer therapeutic, though it has faced clinical setbacks due to off-target effects. To investigate the protein levels changed of HL60 cells (acute myeloid leukemia cell line) by CB-5083 in cytoplasmic, nuclear, and insoluble membrane compartments, we employed fractionation and label-free proteomic analysis. Results reveal distinct compartment-specific protein regulation, providing insight into CB5083’s cellular mechanisms and potential for more targeted therapeutic applications.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:08:03.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting-Yu Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 10:43:22	ID requested
⏵ 1	2025-05-07 03:08:04	announced

10.17912/micropub.biology.001372;

Huang W, Qiu Y, Huynh D, Wang TY, Chou TF, Proteomics analysis reveals the differential impact of the p97 inhibitor CB-5083 on protein levels in various cellular compartments of the HL-60 cell line. MicroPubl Biol, 2024():(2024) [pubmed]

submitter keyword: p97 VCP, p97 inhibitor

mass spectrometry., CB-5083

Tsui-Fen Chou
contact affiliation	Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology
contact email	tfchou@caltech.edu
lab head
Ting-Yu Wang
contact affiliation	Caltech
contact email	tywang@caltech.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056029

PRIDE project URI

• PRIDE
  1. PXD056029
     1. Label: PRIDE project
     2. Name: Differential Impact of p97 Inhibitor CB-5083 on Protein Dynamics in Nucleus, Cytoplasm, and Membrane Compartments