⮝ Full datasets listing
PXD056028
PXD056028 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Wnt signaling inhibits Casein kinase 1a activity by modulating its interaction with Protein phosphatase 2A |
| Description | The mechanism by which Wnt signaling, an essential pathway controlling development and disease, stabilizes beta-Catenin has been a subject of debate over the last three decades. Casein kinase 1alpha (CK1a) functions as a pivotal negative regulator of this signaling pathway, initiating the events that destabilize beta-Catenin. However, whether and how CK1a activity is regulated in Wnt-off and Wnt-on states remains poorly understood. We now show that CK1a activity requires its association with the alpha catalytic subunit of Protein phosphatase 2A (PPP2CA) on AXIN, the scaffold protein of the beta-Catenin destruction complex. Wnt stimulation induces the dissociation of PPP2CA from CK1a, resulting in CK1a autophosphorylation and its consequent inactivation. Moreover, autophosphorylated CK1a is enriched in a subset of colorectal cancers (CRC) harboring constitutive Wnt activation. Our findings identify a novel mechanism by which Wnt stimulation inactivates CK1a, filling a critical gap in our understanding of Wnt signaling, with relevance for CRC. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-20 |
| AnnouncementXML | Submission_2026-02-20_11:07:37.520.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hsin-Yao Tang |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-09-19 09:56:04 | ID requested | |
| ⏵ 1 | 2026-02-20 11:07:38 | announced |
Publication List
| Shen C, Lu W, Merugu SB, Bharti A, Afify SM, Schnitkey L, Wynn DT, Yang F, Rohwetter TM, Nayak A, Bunnag N, Cywiak C, Tang HY, Harris BT, Albanese C, Ihemelandu C, Cobb MH, Kettenbach A, Lee E, Ahmed Y, Robbins DJ, activity by modulating its interaction with protein phosphatase 2A. Cell Rep, 44(2):115274(2025) [pubmed] |
Keyword List
| submitter keyword: CK1a, Wnt signaling, Protein phosphatase 2A, autophosphorylation, colorectal cancer |
Contact List
| David J. Robbins | |
|---|---|
| contact affiliation | Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University |
| contact email | dr956@georgetown.edu |
| lab head | |
| Hsin-Yao Tang | |
| contact affiliation | The Wistar Institute |
| contact email | tangh@wistar.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095904/ |
