PXD056027

PXD056027 is an original dataset announced via ProteomeXchange.

Title	Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - AAK1 C-terminal interactome profiling – batch 1
Description	NAKs (Numb-associated kinases) are a relatively unexplored kinase family that interacts with the endocytic machinery. Among NAK members, AAK1 has been linked to disorders such as neuropathic pain, schizophrenia, and Alzheimer's disease. Our goal is to better understand the cellular pathways regulated by AAK1 as its only known role until now has been in endocytosis. Using a synthetic peptide identical to the C-terminus of AAK1, we observed an interaction with both the clathrin heavy chain and a subset of proteins containing the PDZ domain, including the newly identified AAK1 substrate, PDLIM5. This finding is significant as it reveals that AAK1 and PDLIM5 interact not only through the kinase domain of AAK1 and PDLIM5's consensus motif surrounding the threonine 290 (T290) but also through the PDZ domain of PDLIM5 binding the LIDL motif at the C-terminus of AAK1.
HostingRepository	PRIDE
AnnounceDate	2026-03-19
AnnouncementXML	Submission_2026-03-19_08:28:32.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 09:20:38	ID requested
⏵ 1	2026-03-19 08:28:33	announced

Dataset with its publication pending

submitter keyword: LC-MS, DIA, co-immunoprecipitation, AAK1, interactomics

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD056027

PRIDE project URI

• PRIDE
  1. PXD056027
     1. Label: PRIDE project
     2. Name: Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - AAK1 C-terminal interactome profiling – batch 1