PXD056024

PXD056024 is an original dataset announced via ProteomeXchange.

Title	Metalloproteome factory: from crude extract to identification of metalloproteins via 3-dimensional HPLC-ICP-sfMS
Description	Metalloproteins are essential for a vast array of biological processes in all living organisms. Identification of multiple metalloproteins from native expression (“metalloproteomics”) based on a single chromatography has low resolution and/or sensitivity, while classical protein purification lacks the versatility required for purifying multiple proteins for proteomics. We present a new metalloproteomics method based on three dimensions of liquid chromatography hyphenated with inductively coupled sector-field mass spectrometry (ICP-sfMS): anion exchange chromatography (AEC) followed by either chromatofocusing (CF) or hydrophobic interaction chromatography (HIC), and finally size exclusion chromatography (SEC). We used this 3D technique to purify naturally abundant metalloproteins from crude (mainly) plant, bacteria and animal extracts to a quantity and purity sufficient for protein identification and quantification by mass spectrometry (Tims-TOF-MS). Sensitivity and signal/background ratio were sufficient to identify proteins at concentrations < 1 nM and total amounts < 1 pM in the final SEC.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_19:49:25.245.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Filip Dyčka
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847; scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 08:47:57	ID requested
⏵ 1	2025-08-24 19:49:26	announced

K, ü, pper H, Bokhari SNH, Konik P, Dy, č, ka F, Flores-Sanchez IJ, Andresen E, Jaime-P, é, rez N, Colussi A, Morina F, Metalloproteome Factory: From Crude Extract to Identification of Metalloproteins via 3-Dimensional HPLC-ICP-MS. Anal Chem, 97(32):17641-17649(2025) [pubmed]

10.1021/acs.analchem.5c02775;

submitter keyword: metal-free high performance liquid chromatography, metallomics,ICP-MS, HPLC, metalloproteomics, ultra-trace protein analysis

Hendrik Küpper
contact affiliation	1. Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Laboratory of Plant Biophysics & Biochemistry, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic 2. University of South Bohemia, Faculty of Science, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic
contact email	hendrik.kuepper@umbr.cas.cz
lab head
Filip Dyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056024
     1. Label: PRIDE project
     2. Name: Metalloproteome factory: from crude extract to identification of metalloproteins via 3-dimensional HPLC-ICP-sfMS