PXD056021

PXD056021 is an original dataset announced via ProteomeXchange.

Title	Non-hydrolyzable acetyllysine analogs to study protein acetylation in vitro and in cells
Description	Lysine acetylation plays a prominent regulatory role in eukaryotic cells. Yet, determining the functional consequences of acetylation for a given protein represents a considerable challenge. For instance, lysine residues are subject to various posttranslational modifications, rendering interpretation of mutational studies difficult. The genetic code expansion technology enables the site-specific incorporation of acetyllysine (AcK) into proteins, but the applicability of AcK is limited, as within cells, the acetyl group is removed by deacetylases. Here, we show that site-specific incorporation of the non-hydrolyzable AcK analog ketolysine (KeK) into ubiquitin closely resembles the structural and functional effects of AcK incorporation. Furthermore, AcK and KeK can be efficiently incorporated into the tumor suppressor p53. However, whereas AcK becomes deacetylated, KeK remains stable. Accordingly, incorporation of KeK, but not AcK, affects p53-mediated transcription. Thus, we propose that KeK is the AcK surrogate of choice for studying protein acetylation in cells.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_22:16:35.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 07:39:34	ID requested
⏵ 1	2026-02-22 22:16:36	announced

10.1038/S41467-026-69782-6;

submitter keyword: AP-MS, PRM, PTM,Acetylation, ubiquitin, p53, KeK, genetic code expansion

Prof. Dr. Florian Stengel
contact affiliation	Department of Biology, University of Konstanz, Universitaetsstrasse 10, 78457 Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056021
     1. Label: PRIDE project
     2. Name: Non-hydrolyzable acetyllysine analogs to study protein acetylation in vitro and in cells