PXD056016

PXD056016 is an original dataset announced via ProteomeXchange.

Title	The impact of hypoxia on KGN cells
Description	Human granulosa cells (GCs) give rise to granulosa cell tumors (GCTs), in which a hypoxic situation can be assumed. KGN cells are a widely used cellular model for both tumorous and normal human GCs. In this study, we evaluated consequences of hypoxic (1% O2) versus atmospheric conditions on KGNs for a total of 10 days. A proteomic analysis showed 57 more and 75 less abundant proteins in hypoxia. Of these proteins, 72 overlap with the ones of GCs exposed to hypoxia (ProteomeXchange Consortium dataset identifier PXD042934). Common up-regulated pathways include cellular response to hypoxia and glucose metabolism, whereas the mitochondrial 5’-end processing pathway was significantly down-regulated in both cell types. 60 proteins were uniquely regulated in KGN cells, indicating cell-type-specific responses. This is in line with findings of nuclear expression of HIF1α in acute (2h) and prolonged (10d) hypoxia in KGNs, in contrast to its nuclear presence in GCs only in acute hypoxia. Hypoxia also affected cell number and size but not the migratory behavior of KGNs. The results provide a detailed picture of hypoxia-induced changes in KGNs and indicate that low O2 in the tumor microenvironment may have an important and multifaceted influence on GCTs.
HostingRepository	PRIDE
AnnounceDate	2025-11-19
AnnouncementXML	Submission_2025-11-19_04:23:42.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 05:53:02	ID requested
⏵ 1	2025-11-19 04:23:43	announced

10.1530/raf-25-0017;

H, ö, fner M, Schneider M, Jiang Y, Schneider J, Herrmann C, Mayr D, Forn, é I, Mayerhofer A, The impact of hypoxia on the cellular phenotype and proteome of KGN cells. Reprod Fertil, 6(4):(2025) [pubmed]

submitter keyword: proteomics, hypoxia,Granulosa cell tumor

Prof. Dr. med. Artur Mayerhofer
contact affiliation	Department for Cell Biology BioMedizinisches Centrum - BMC LMU Muenchen, Med. Faculty Großhaderner Straße 9 82152 Planegg-Martinsried
contact email	mayerhofer@lrz.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD056016

PRIDE project URI

• PRIDE
  1. PXD056016
     1. Label: PRIDE project
     2. Name: The impact of hypoxia on KGN cells