PXD056003

PXD056003 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Cotton Leaves Under Heat Stress
Description	Cotton (Gossypium hirsutum L.), a crucial global fiber and oil seed crop, faces a diverse biotic and abiotic stresses. Among these, temperature stress strongly influence its growth, prompting adaptive physiological, biochemical and molecular changes. In this study, we explored the proteomic changes underscoring the heat stress tolerance in the leaves of two locally developed cotton genotypes, i.e., heat tolerant (GH-Hamaliya Htol) and heat susceptible (CIM-789 Hsus), guided by morpho-physiological and biochemical analysis. These genotypes were sown at two different temperatures, control (35ºC) and stress (45ºC) in glass house, in randomized complete block design (RCBD) in three replications. At the flowering stage, a label-free quantitative shotgun proteomics of cotton leaves revealed the differential expression of 701 and 1270 proteins in the tolerant and susceptible genotype compared to the control, respectively. Physiological and biochemical analysis showed that the heat-tolerant genotype responded uniquely to stress by maintaining the net photosynthetic rate (Pn)( 25.2-17.5µmolCO2m-2S-1), chlorophyll (8.5-7.8mg/g FW), and proline contents (4.9-7.4 µmole/g) compared to control, supported by the upregulation of many proteins involved in several pathways including photosynthesis, oxidoreductase activity, response to stresses, translation, transporter activities as well asprotein and carbohydrate metabolic processes. In contrast, the distinctive pattern of protein downregulation involved in stress response, oxidoreductase activity, and carbohydrate metabolism was observed in susceptible plants. Thus, in this study, the specific proteins that increased in abundance under heat stress tolerance mechanism can be used as markers in future for producing the heat tolerant cotton genotypes without compromising the yield.
HostingRepository	PRIDE
AnnounceDate	2025-01-13
AnnouncementXML	Submission_2025-01-13_03:10:16.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohibullah Shah
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 22:46:28	ID requested
⏵ 1	2025-01-13 03:10:16	announced

10.1371/JOURNAL.PONE.0316630;

submitter keyword: Thermo-tolerance, Label-free proteomics, Protein biomarkers, Shotgun proteomics

Asia Perveen
contact affiliation	Student
contact email	asiaahs@yahoo.com
lab head
Mohibullah Shah
contact affiliation	Bahauddin Zakariya University
contact email	mohib@bzu.edu.pk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056003
     1. Label: PRIDE project
     2. Name: Proteomics of Cotton Leaves Under Heat Stress