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PXD055987

PXD055987 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli
DescriptionPeatlands play a crucial role in global climate protection as carbon sinks and their rewetting is increasingly significant but poses economic challenges for agriculture. A promising application for rewetted peatlands is the cultivation of Drosera rotundifolia L., a medicinal plant with potential for sustainable phytopharmaceutical development. The plant's bioactive compounds, particularly flavonoids and naphthoquinones, exhibit biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains, offering new therapeutic options. This study investigates the molecular mechanisms of these compounds in biofilm inhibition through proteomic analyses. Specific fractions of flavonoids and naphthoquinones, as well as individual substances like 7-methyl juglone and 2’’-O-galloyl hyperoside, are analyzed. Results show that flavonoids from Drosera rotundifolia L. likely affect biofilm formation by creating an iron-poor environment through iron complexation and additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations include assays for iron complexation and analysis of polyamines confirmed the proteomic data. In-silico docking studies identify potential molecular targets of the bioactive compounds. Safety evaluations through cytotoxicity tests in 3D cell cultures and the Galleria mellonella in-vivo model confirmed the safety of the extracts used. These findings highlight Drosera rotundifolia L. as a promising candidate for new phytopharmaceuticals and support the sustainable use of rewetted peatlands.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_03:13:00.901.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandra Maass
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListacetylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-18 08:26:18ID requested
12025-05-07 03:13:01announced
Publication List
10.1016/j.bioflm.2025.100268;
Gerschler S, Maa, ß S, Gerth P, Schulig L, Wildgrube T, Rockstroh J, Wurster M, Methling K, Becher D, Lalk M, Schulze C, Guenther S, Schultze N, biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies. Biofilm, 9():100268(2025) [pubmed]
Keyword List
submitter keyword: drosera rotundifolia, 3D cell culture model, flavonoids, mode of action, polyamines, naphthoquinones, iron, cytotoxicity
Contact List
Dörte Becher
contact affiliationUniversity of Greifswald, Institute of Microbiology, 17489 Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliationUniversity of Greifswald, Department for Microbial Proteomics
contact emailsandra.maass@uni-greifswald.de
dataset submitter
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Dataset FTP location
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