PXD055987

PXD055987 is an original dataset announced via ProteomeXchange.

Title	Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli
Description	Peatlands play a crucial role in global climate protection as carbon sinks and their rewetting is increasingly significant but poses economic challenges for agriculture. A promising application for rewetted peatlands is the cultivation of Drosera rotundifolia L., a medicinal plant with potential for sustainable phytopharmaceutical development. The plant's bioactive compounds, particularly flavonoids and naphthoquinones, exhibit biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains, offering new therapeutic options. This study investigates the molecular mechanisms of these compounds in biofilm inhibition through proteomic analyses. Specific fractions of flavonoids and naphthoquinones, as well as individual substances like 7-methyl juglone and 2’’-O-galloyl hyperoside, are analyzed. Results show that flavonoids from Drosera rotundifolia L. likely affect biofilm formation by creating an iron-poor environment through iron complexation and additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations include assays for iron complexation and analysis of polyamines confirmed the proteomic data. In-silico docking studies identify potential molecular targets of the bioactive compounds. Safety evaluations through cytotoxicity tests in 3D cell cultures and the Galleria mellonella in-vivo model confirmed the safety of the extracts used. These findings highlight Drosera rotundifolia L. as a promising candidate for new phytopharmaceuticals and support the sustainable use of rewetted peatlands.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:13:00.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 08:26:18	ID requested
⏵ 1	2025-05-07 03:13:01	announced

10.1016/j.bioflm.2025.100268;

Gerschler S, Maa, ß S, Gerth P, Schulig L, Wildgrube T, Rockstroh J, Wurster M, Methling K, Becher D, Lalk M, Schulze C, Guenther S, Schultze N, biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies. Biofilm, 9():100268(2025) [pubmed]

submitter keyword: drosera rotundifolia, 3D cell culture model, flavonoids, mode of action, polyamines, naphthoquinones, iron, cytotoxicity

Dörte Becher
contact affiliation	University of Greifswald, Institute of Microbiology, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055987
     1. Label: PRIDE project
     2. Name: Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli