PXD055987 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli |
Description | Peatlands play a crucial role in global climate protection as carbon sinks and their rewetting is increasingly significant but poses economic challenges for agriculture. A promising application for rewetted peatlands is the cultivation of Drosera rotundifolia L., a medicinal plant with potential for sustainable phytopharmaceutical development. The plant's bioactive compounds, particularly flavonoids and naphthoquinones, exhibit biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains, offering new therapeutic options. This study investigates the molecular mechanisms of these compounds in biofilm inhibition through proteomic analyses. Specific fractions of flavonoids and naphthoquinones, as well as individual substances like 7-methyl juglone and 2’’-O-galloyl hyperoside, are analyzed. Results show that flavonoids from Drosera rotundifolia L. likely affect biofilm formation by creating an iron-poor environment through iron complexation and additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations include assays for iron complexation and analysis of polyamines confirmed the proteomic data. In-silico docking studies identify potential molecular targets of the bioactive compounds. Safety evaluations through cytotoxicity tests in 3D cell cultures and the Galleria mellonella in-vivo model confirmed the safety of the extracts used. These findings highlight Drosera rotundifolia L. as a promising candidate for new phytopharmaceuticals and support the sustainable use of rewetted peatlands. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_03:13:00.901.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandra Maass |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-18 08:26:18 | ID requested | |
⏵ 1 | 2025-05-07 03:13:01 | announced | |
Publication List
10.1016/j.bioflm.2025.100268; |
Gerschler S, Maa, ß S, Gerth P, Schulig L, Wildgrube T, Rockstroh J, Wurster M, Methling K, Becher D, Lalk M, Schulze C, Guenther S, Schultze N, biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies. Biofilm, 9():100268(2025) [pubmed] |
Keyword List
submitter keyword: drosera rotundifolia, 3D cell culture model, flavonoids, mode of action, polyamines, naphthoquinones, iron, cytotoxicity |
Contact List
Dörte Becher |
contact affiliation | University of Greifswald, Institute of Microbiology, 17489 Greifswald, Germany |
contact email | dbecher@uni-greifswald.de |
lab head | |
Sandra Maass |
contact affiliation | University of Greifswald,
Department for Microbial Proteomics |
contact email | sandra.maass@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055987
- Label: PRIDE project
- Name: Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli