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PXD055978

PXD055978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
DescriptionWe developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest populations formed after endocytic vesicle uncoating. We demonstrated its utility by revealing the endolysosomal protein landscapes for cortical-like iNeurons and stem cells. This allowed us to globally profile endocytic cargo, identifying hundreds of transmembrane proteins.
HostingRepositoryPRIDE
AnnounceDate2025-01-11
AnnouncementXMLSubmission_2025-01-11_14:50:12.362.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD055978
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMiguel A. Gonzalez-Lozano
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-18 06:56:40ID requested
12025-01-11 14:50:13announced
Publication List
10.1073/pnas.2419079121;
10.6019/PXD055978;
Hundley FV, Gonzalez-Lozano MA, Gottschalk LM, Cook ANK, Zhang J, Paulo JA, Harper JW, Endo-IP and lyso-IP toolkit for endolysosomal profiling of human-induced neurons. Proc Natl Acad Sci U S A, 121(52):e2419079121(2024) [pubmed]
Keyword List
submitter keyword: Human, TMT, Endosomes, iNeurons, Lysosomes
Contact List
J. Wade Harper
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston MA
contact emailwade_harper@hms.harvard.edu
lab head
Miguel A. Gonzalez-Lozano
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston MA
contact emailmiguel_gonzalezlozano@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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