PXD055978 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons |
Description | We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest populations formed after endocytic vesicle uncoating. We demonstrated its utility by revealing the endolysosomal protein landscapes for cortical-like iNeurons and stem cells. This allowed us to globally profile endocytic cargo, identifying hundreds of transmembrane proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-11 |
AnnouncementXML | Submission_2025-01-11_14:50:12.362.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055978 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Miguel A. Gonzalez-Lozano |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-18 06:56:40 | ID requested | |
⏵ 1 | 2025-01-11 14:50:13 | announced | |
Publication List
10.1073/pnas.2419079121; |
10.6019/PXD055978; |
Hundley FV, Gonzalez-Lozano MA, Gottschalk LM, Cook ANK, Zhang J, Paulo JA, Harper JW, Endo-IP and lyso-IP toolkit for endolysosomal profiling of human-induced neurons. Proc Natl Acad Sci U S A, 121(52):e2419079121(2024) [pubmed] |
Keyword List
submitter keyword: Human, TMT, Endosomes, iNeurons, Lysosomes |
Contact List
J. Wade Harper |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | wade_harper@hms.harvard.edu |
lab head | |
Miguel A. Gonzalez-Lozano |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | miguel_gonzalezlozano@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD055978 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055978
- Label: PRIDE project
- Name: Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons