PXD055978

PXD055978 is an original dataset announced via ProteomeXchange.

Title	Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
Description	We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest populations formed after endocytic vesicle uncoating. We demonstrated its utility by revealing the endolysosomal protein landscapes for cortical-like iNeurons and stem cells. This allowed us to globally profile endocytic cargo, identifying hundreds of transmembrane proteins.
HostingRepository	PRIDE
AnnounceDate	2025-01-11
AnnouncementXML	Submission_2025-01-11_14:50:12.362.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055978
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Miguel A. Gonzalez-Lozano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 06:56:40	ID requested
⏵ 1	2025-01-11 14:50:13	announced

10.1073/pnas.2419079121;

10.6019/PXD055978;

Hundley FV, Gonzalez-Lozano MA, Gottschalk LM, Cook ANK, Zhang J, Paulo JA, Harper JW, Endo-IP and lyso-IP toolkit for endolysosomal profiling of human-induced neurons. Proc Natl Acad Sci U S A, 121(52):e2419079121(2024) [pubmed]

submitter keyword: Human, TMT, Endosomes, iNeurons, Lysosomes

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	wade_harper@hms.harvard.edu
lab head
Miguel A. Gonzalez-Lozano
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	miguel_gonzalezlozano@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD055978

PRIDE project URI

• PRIDE
  1. PXD055978
     1. Label: PRIDE project
     2. Name: Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons