PXD055899

PXD055899 is an original dataset announced via ProteomeXchange.

Title	Selective Modification of Asparagine and Glutamine via Isohypsic Reaction
Description	The selective modification of amino acids within proteins represents the epitome of a chemoselective reaction. Current protein modification techniques are constrained to nucleophilic and redox-active residues, restricting their ability to target amino acids outside this scope. Herein, we introduce the method for selectively modifying Asparagine and Glutamine in proteins that have never been targeted before via unorthodox isohypsic reaction to bioorthogonal nitrile handles under physiological conditions. This methodology not only expands the scope of protein modification but also unveils new, ligandable sites across the human proteome through comprehensive chemoproteomic profiling. Additionally, the isohypsic dehydration reaction has been effectively employed to profile deamidation and N-glycosylation post-translational modifications on Asparagine and Glutamine, leading to the identification of previously unknown deamidation sites and significant alterations in N-glycosylation events linked to disease processes. This research offers a transformative approach with profound implications for protein engineering and understanding disease mechanisms.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_18:51:22.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Beusch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 07:06:13	ID requested
⏵ 1	2026-05-24 18:51:23	announced

10.1021/acschembio.6c00173;

Emenike B, Talbott JM, Paikin ZE, Beusch CM, Khoshnevis S, Gordon DE, Raj M, Expanding the Chemoproteomic Toolkit to Asparagine and Glutamine. ACS Chem Biol, 21(5):1189-1198(2026) [pubmed]

submitter keyword: Asparagine, PTM,chemical proteomics, Glutamine

David Ezra Gordon
contact affiliation	Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, United States
contact email	david.ezra.gordon@emory.edu
lab head
Christian Beusch
contact affiliation	Emory University
contact email	christian.beusch@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD055899

PRIDE project URI

• PRIDE
  1. PXD055899
     1. Label: PRIDE project
     2. Name: Selective Modification of Asparagine and Glutamine via Isohypsic Reaction