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PXD055898

PXD055898 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructure of a Gcn2 dimer in complex with the large 60S ribosomal subunit
DescriptionThe integrated stress response (ISR) is a central signalling network that enables eukaryotic cells to respond to a variety of different environmental stresses. Such stresses cause ribosome collisions that lead to activation of the kinase Gcn2, resulting in the phosphorylation and inactivation of eukaryotic initiation factor 2 and thereby promoting selective translation of mRNAs to restore homeostasis. Despite the importance of the ISR and intensive study over the past decades, structural insight into how Gcn2 interacts with ribosomal particles has been lacking. Using ex vivo affinity purification approaches, we have obtained a native structure of a Gcn2 dimer in complex with the ribosomal 60S subunit. The Gcn2 dimer is formed by dimerization of the histidine tRNA synthetase-like domains, which establish extensive interactions with the stalk-base and sarcin-ricin loop of the 60S subunit. The C-terminal domain of Gcn2 is also dimerized and occupies the A- and P-site tRNA binding sites at the peptidyl-transferase center of the 60S subunit. Complementary functional studies indicate that binding of Gcn2 to the 60S subunit does not require the co-activators Gcn1 or Gcn20, nor does it lead to phosphorylation of eIF2a. Rather, upon stress, we observe a shift of Gcn2 from the 60S subunit into the colliding ribosome fraction, suggesting that the Gcn2-60S complex represents an inactive stand-by state to enable a rapid redistribution to collided ribosomes, and thereby facilitating a quick and efficient response to stress.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:58:26.505.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTanel Tenson
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-16 06:51:07ID requested
12024-09-16 07:05:51announced
22024-10-22 06:58:26announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ribosome, cryoEM
Contact List
Tanel Tenson
contact affiliationInstitute of Technology, University of Tartu, Tartu 50411, Estonia
contact emailtanel.tenson@ut.ee
lab head
Tanel Tenson
contact affiliationUniversity of Tartu
contact emailtanel.tenson@ut.ee
dataset submitter
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Dataset FTP location
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