PXD055895

PXD055895 is an original dataset announced via ProteomeXchange.

Title	An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters
Description	In all three domains of life, genes with related functions can be enriched in specific genomic regions, known as the gene clusters. In eukaryotes, histone, piRNA, and rDNA (ribosomal DNA) clusters are among the most notable clusters which play fundamental roles in chromatin formation, genome integrity, and translation, respectively. These clusters have traditionally been thought to be regulated by distinct transcriptional mechanisms. In this study, using C. elegans as a model system we identify ATTF-6, a member of the AT-hook family, as a key factor for the expression of histone, piRNA, and 5S rDNA-SL1 (spliced leader 1) clusters. ATTF-6 forms distinct nuclear foci at both piRNA and 5S rDNA-SL1 clusters. Loss of ATTF-6 leads to a depletion of histone mRNAs and SL1 transcripts. Additionally, we demonstrate that ATTF-6 is required for the recruitment of USTC (Upstream Sequence Transcription Complex) at piRNA clusters, which is necessary for piRNA production. Collectively, our findings underscore the essential role of an AT-hook transcription factor in promoting the expression of fundamental gene clusters.
HostingRepository	PRIDE
AnnounceDate	2025-01-29
AnnouncementXML	Submission_2025-01-29_06:10:41.877.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055895
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	WEN TANG
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 06:14:38	ID requested
⏵ 1	2025-01-29 06:10:42	announced

10.6019/PXD055895;

submitter keyword: histone, spliced-leader,AT-hook transcription factor, piRNA clusters

Wen Tang
contact affiliation	Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH 43210, USA. Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
contact email	tang.542@osu.edu
lab head
WEN TANG
contact affiliation	Ohio State University
contact email	tang.542@osu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD055895

PRIDE project URI

• PRIDE
  1. PXD055895
     1. Label: PRIDE project
     2. Name: An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters