PXD055874

PXD055874 is an original dataset announced via ProteomeXchange.

Title	Mouse Prefontal Cortex Astrocyte Proteome
Description	Anxiety disorders are among the most common and disabling mental health disorders worldwide with inadequate therapeutic options. Numerous clinical and research studies indicate essential neuronal contribution to anxiety, yet the mechanisms of non-neuronal cells governing this emotional state remain poorly understood. Here, we show that non-neuronal astrocytes in the medial prefrontal cortex (mPFC) encode anxiogenic environmental cues. Silencing mPFC astrocyte Ca2+ signaling heightens anxiety-like behavior and disrupts the balance between excitatory and inhibitory neuronal population activity. Moreover, this effect is differentially pronounced across behaviorally tuned neuronal subpopulations on single cell and network levels. Using cell type-specific proximity-dependent biotinylation approaches, we further identified significant protein expression changes in astrocytes and at the astrocyte-neuron interface within the mPFC.
HostingRepository	PRIDE
AnnounceDate	2025-07-13
AnnouncementXML	Submission_2025-07-13_14:38:26.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanqi Tan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-15 15:18:59	ID requested
⏵ 1	2025-07-13 14:38:26	announced

Dataset with its publication pending

submitter keyword: Astrocyte, LCMS

Jonathan Sweedler
contact affiliation	University of Illinois Urbana Champaign
contact email	jsweedle@illinois.edu
lab head
Yanqi Tan
contact affiliation	University of Illinois Urbana Champaign
contact email	tan61@illinois.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055874

PRIDE project URI

• PRIDE
  1. PXD055874
     1. Label: PRIDE project
     2. Name: Mouse Prefontal Cortex Astrocyte Proteome