PXD055873

PXD055873 is an original dataset announced via ProteomeXchange.

Title	Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - AAK1 differential interactome profiling
Description	NAKs (Numb-associated kinases) are a relatively unexplored kinase family that interacts with the endocytic machinery. Among NAK members, AAK1 has been linked to disorders such as neuropathic pain, schizophrenia, and Alzheimer's disease. Our goal is to better understand the cellular pathways regulated by AAK1 as its only known role until now has been in endocytosis. To define the AAK1 interactome in relation to focal adhesions, we compared its interactome in cells plated for 2 hours on collagen, which promotes focal adhesions, and poly-L-lysine (PLL), which does not. In collagen-plated cells, we discovered several differential AAK1 interactors involved in focal adhesions, the actin cytoskeleton, and recycling endosomes. In PLL-plated cells, there was a significant increase in actin cytoskeleton components and upregulation of the entire Arp2/3 complex as AAK1 interactors. This supports our other data showing that AAK1 is involved in focal adhesion maintenance and cell migration.
HostingRepository	PRIDE
AnnounceDate	2026-03-19
AnnouncementXML	Submission_2026-03-19_09:52:36.496.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-15 11:15:47	ID requested
⏵ 1	2026-03-19 09:52:36	announced

Dataset with its publication pending

submitter keyword: LC-MS, DIA, co-immunoprecipitation, AAK1, interactomics

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD055873

PRIDE project URI

• PRIDE
  1. PXD055873
     1. Label: PRIDE project
     2. Name: Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - AAK1 differential interactome profiling