PXD055869

PXD055869 is an original dataset announced via ProteomeXchange.

Title	Establishment of Salivary Tissue-Organoid Biorepository
Description	The salivary gland plays a crucial role in oral function and overall health by secreting saliva, yet salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and PSMA- phenotype of both submandibular and parotid glands, with clonal differentiation assays highlighting heterogeneity. Induction of PSMA/FOLH1 expression was associated with SGSPC differentiation. Utilizing label-free single-cell proteomics, we identified 819 unique proteins in SGSPC-enriched cells, including co-expressed cytokeratins, expressed in rare salivary ductal basal cells. Additionally, PRDX, a unique class of peroxiredoxin peroxidases enriched in SGSPCs demonstrated H2O2-dependent growth, suggesting a role in salivary homeostasis. These findings lay a solid foundation for studying SGSPCs and may lead to personalized regenerative medicine for salivary gland dysfunction.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_18:24:55.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-15 02:39:49	ID requested
⏵ 1	2025-05-25 18:24:56	announced

Aalam SMM, Varela AR, Khaderi A, Mondesir RJ, Mun DG, Ding A, Lombaert IMA, Coppes RP, Emperumal CP, Pandey A, Janus JR, Kannan N, Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1. NPJ Regen Med, 10(1):23(2025) [pubmed]

10.1038/s41536-025-00410-5;

submitter keyword: salivary gland, single cell proteomics, biobanking, scRNA sequencing

Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
contact email	Pandey.akhilesh@mayo.edu
lab head
Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905
contact email	pandey.akhilesh@mayo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055869

PRIDE project URI

• PRIDE
  1. PXD055869
     1. Label: PRIDE project
     2. Name: Establishment of Salivary Tissue-Organoid Biorepository