PXD055858 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ERH regulates type II interferon immune signaling through post-transcriptional regulation of JAK2 mRNA |
Description | Type II interferon (IFNγ) signaling is essential for innate immunity and critical for effective immunological checkpoint blockade in cancer immunotherapy. Genetic screen identification of post-transcriptional regulators of this pathway has been challenging since such factors are often essential for cell viability. Here, we utilize our inducible CRISPR/Cas9 approach to screen for key post-transcriptional regulators of IFNγ signaling, and in this way identify ERH and the ERH-associated splicing and RNA export factors MAGOH, SRSF1, and ALYREF. Loss of these factors impairs post- transcriptional mRNA maturation of JAK2, a crucial kinase for IFNγ signaling, resulting in abrogated JAK2 protein levels and diminished IFNγ signaling. Further analysis highlights a critical role for ERH in preventing intron retention in AU-rich regions in specific transcripts, such as JAK2. This regulation is markedly different from previously described retention of GC-rich introns. Overall, these findings reveal that post transcriptional JAK2 processing is a critical rate-limiting step for the IFNγ-driven innate immune response. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-30 |
AnnouncementXML | Submission_2025-05-30_08:57:18.715.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | F Butter |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-13 14:55:32 | ID requested | |
⏵ 1 | 2025-05-30 08:57:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS |
Contact List
Falk Butter |
contact affiliation | Institute of Molecular Biology |
contact email | f.butter@imb.de |
lab head | |
F Butter |
contact affiliation | Quantitative Proteomics
Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055858
- Label: PRIDE project
- Name: ERH regulates type II interferon immune signaling through post-transcriptional regulation of JAK2 mRNA