PXD055850
PXD055850 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Acquisition of embryogenic competence during protoplast development of Fagopyrum |
| Description | Reprogramming in protoplast cultures is a key step for acquiring embryogenic competence by dedifferentiated cells, thus leading to plant regeneration. Therefore, to better understand factors trigging the processes, this work focuses on analysing proteomes, cell wall composition and changes in the expression profile of selected genes. The results demonstrate a significant accumulation of somatic embryogenesis related proteins like late embryogenesis abundant proteins (embryogenic protein-DC-8-like, seed biotin-containing protein) and endochitinases during the developmental path of protoplast cultures supporting somatic embryogenesis events. Additionally, there is an increase in seed storage proteins like vicilin, oleosins, and seed biotin-containing proteins in the cultures. Investigation of somatic embryogenesis-associated transcription factors revealed intensive up-regulation of LEC1 for 50th day of F. tataricum protoplast cultures. Furthermore, we demonstrated the variable distribution of cell wall components like pectin side chains, arabinogalactan proteins (AGP) and extansin (EXT), indicating the acquisition of competence and cellular differentiation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-06-25 |
| AnnouncementXML | Submission_2025-06-25_02:16:37.795.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Urszula Jankowska |
| SpeciesList | scientific name: Fagopyrum tataricum; common name: Tartarian buckwheat; NCBI TaxID: 62330; scientific name: Fagopyrum esculentum; common name: common buckwheat; NCBI TaxID: 3617; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-09-13 07:21:03 | ID requested | |
| ⏵ 1 | 2025-06-25 02:16:38 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: differentiation, embryogenic competence, Fagopyrum, oleosins, protoplast cultures, somatic embryogenesis, vicilins |
Contact List
| Artur Pinski | |
|---|---|
| contact affiliation | Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice |
| contact email | artur.pinski@us.edu.pl |
| lab head | |
| Alexander Betekhtin | |
| contact affiliation | Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice |
| contact email | alexander.betekhtin@us.edu.pl |
| lab head | |
| Urszula Jankowska | |
| contact affiliation | Jagiellonian University |
| contact email | urszula.jankowska@uj.edu.pl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095861/ |
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