PXD055840

PXD055840 is an original dataset announced via ProteomeXchange.

Title	The use of three-dimensional primary human myospheres to explore skeletal muscle effects of in vivo krill oil supplementation
Description	Supplementation with krill oil has shown effects on whole-body lipid and glucose metabolism, as well as on skeletal muscle strength and function. The most widely used model for culturing skeletal muscle cells (myotubes) is two-dimensional (2D), however there is growing interest in establishing 3D muscle cell models. We previously showed that intervention with krill oil in vivo promoted increased fatty acid metabolism and protein synthesis in cultured human myotubes in a 2D model. The aim of this study was to explore the effects of krill oil supplementation in vivo in a 3D myosphere model to evaluate the usefulness of a human skeletal 3D model compared to established 2D models. Myospheres were formed from myoblasts obtained before and after 7 weeks of in vivo krill oil intervention. Glucose and oleic acid metabolism were assessed, and transcriptomic and proteomic analyses were also performed. In contrast to previous data found in 2D myotube cultures, in vivo intervention with krill oil increased glucose metabolism in myospheres, while no effect was observed on fatty acid metabolism. Transcriptomic and proteomic analyses in myospheres obtained before and after krill oil supplementation showed differentially expressed genes/proteins involved in pathways like motor proteins, hypertrophy, calcium signaling and glycolysis/gluconeogenesis, of which motor proteins and hypertrophy have not been described in 2D myotube cultures. These findings suggested that in vivo krill oil intervention induces a different metabolic effect when comparing 3D and 2D cultures. In addition, in contrast to the 2D model, data obtained with the 3D model showed gene expression changes that are more compatible with previously observed results in vivo concerning skeletal muscle motoric function. Hence, the 3D model might better reflect krill oil-induced modifications in skeletal muscle performance in vivo than the 2D model.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:22:00.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-13 03:08:10	ID requested
⏵ 1	2025-08-03 16:22:01	announced

10.1007/s44164-025-00087-6;

Dalmao-Fernandez A, Katare PB, Bakke HG, Hamarsland H, Ellefsen S, Singh S, Nyman TA, Kase ET, Rustan AC, Thoresen GH, The use of three-dimensional primary human myospheres to explore skeletal muscle effects of in vivo krill oil supplementation. In Vitro Model, 4(2):145-155(2025) [pubmed]

submitter keyword: label-free proteomics, 3D muscle cell model,myosphere, krill oil

Tuula Nyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD055840

PRIDE project URI

• PRIDE
  1. PXD055840
     1. Label: PRIDE project
     2. Name: The use of three-dimensional primary human myospheres to explore skeletal muscle effects of in vivo krill oil supplementation