PXD055834

PXD055834 is an original dataset announced via ProteomeXchange.

Title	CDH11 regulates presynaptic vesicle trafficking and neuronal activity in autism-related brain circuit
Description	The molecular etiology of numerous risk genes for autism spectrum disorder (ASD), including CDH11, remains elusive. We investigated the role of CDH11 in the development of ASD-related behaviors using gene-deficient mice. CDH11 is enriched at synapses in glutamatergic neurons of the anterior cingulate cortex (ACC), which project to the striatum, nucleus accumbens, and amygdala. Ablation of Cdh11 in these neurons during development increases self-grooming and reduces sociability, with decreased neuronal activity in the ACC. Chemogenetic inhibition of ACC glutamatergic neurons recapitulates the over-grooming phenotype, while activation of these neurons mitigates self-grooming in Cdh11-deficient mice. Proteomics of ACC synaptosomes and CDH11 interactomes suggest the involvement of CDH11 in synaptic vesicle trafficking, supported by reduced presynaptic vesicle density at excitatory synapses in Cdh11-deficient mice. These findings highlight the critical role of CDH11 in ASD-related brain circuit development and offer insights into the molecular mechanisms and potential therapeutic targets for ASD.
HostingRepository	PRIDE
AnnounceDate	2025-08-08
AnnouncementXML	Submission_2025-08-08_00:25:43.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Ze
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 21:12:52	ID requested
⏵ 1	2025-08-08 00:25:43	announced

10.1038/S41380-025-03141-7;

submitter keyword: synaptic adhesion,ASD, neuronal activity, proteomics, brain circuit, self-grooming, CDH11, ACC

Xiao-Bing Yuan
contact affiliation	Key Laboratory of Brain Functional Genomics of Shanghai and Ministry of Education, Institute of Brain Functional Genomics, School of Life Science and the Collaborative Innovation Center for Brain Science, East China Normal University, Shanghai, China
contact email	xbyuan@brain.ecnu.edu.cn
lab head
Li Ze
contact affiliation	East China Normal University
contact email	lize201916@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055834
     1. Label: PRIDE project
     2. Name: CDH11 regulates presynaptic vesicle trafficking and neuronal activity in autism-related brain circuit