PXD055832

PXD055832 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS of conditioned media from vascularized tissue with and without cardiac microtissues.
Description	Geometrically controlled cardiac microtissues promote vascularization and reduce inflammation in vitro and in vivo Cardiac tissue engineering faces challenges due to inadequate vascularization and lack of effective strategies to control inflammation post transplantation. . This study explores the benefits of geometrically optimized cardiac microtissues (μtissues) over dispersed cardiomyocytes (CMs) for in vitro and in vivo applications. Microtissues derived from human induced pluripotent stem cell-derived CMs (hiPSC-CMs) and compacted in between two PDMS pillars exhibit cellular alignment, contractile function and serve as suitable tissue building blocks. Constraining μtissue size to 300 µm ensures adequate oxygen diffusion, preventing necrotic core formation. In vivo, μtissues demonstrated improved engraftment, contractility, and vascularization in athymic nude rats, with significantly reduced inflammation and enhanced vessel ingrowth from the omental vasculature. This study highlights the potential of μtissues to advance cardiac tissue viability and function by creating contractile grafts with host endothelial ingrowth, offering a promising approach for cardiac repair and regeneration. When compared to dispersed CMs, μtissues show enhanced vessel network formation, reduced cell death (lower LDH), and decreased cytotoxicity (lower cell-free mtDNA). Cytokine analysis revealed elevated pro-angiogenic factors (PIGF, Endocan, Angiopoietin-2) and reduced inflammatory markers (IL-31 RA, IL-2 R beta, OX40 Ligand) in μtissues compared to dispersed CMs.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:23:27.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Saikali
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 19:52:24	ID requested
⏵ 1	2026-05-24 16:23:28	announced

Zhao Y, Khosravi R, Cheung K, Shen K, Wang Y, Landau S, Okhovatian S, Wu Q, Lu RXZ, Wagner KT, Bodenstein DF, Shawky SA, Vosoughi D, Beroncal EL, Yeager K, Cummins CL, Andreazza AC, Vunjak-Novakovic G, Radisic M, . Cell Biomater, 1(4):(2025) [pubmed]

10.1016/j.celbio.2025.100075;

submitter keyword: cardiomyocytes, myocardium, organ-on-a-chip, vascularization, immune response, bioprinting, tissue engineering

Carolyn L. Cummins
contact affiliation	Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Canada
contact email	carolyn.cummins@utoronto.ca
lab head
Michael Saikali
contact affiliation	University of Toronto
contact email	michael.saikali@mail.utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD055832

PRIDE project URI

• PRIDE
  1. PXD055832
     1. Label: PRIDE project
     2. Name: LC-MS/MS of conditioned media from vascularized tissue with and without cardiac microtissues.