PXD055829

PXD055829 is an original dataset announced via ProteomeXchange.

Title	THE FAM53C/DYRK1A AXIS REGULATES THE G1/S TRANSITION OF THE CELL CYCLE
Description	A growing number of small molecules are becoming available to target the cell cycle machinery for the treatment of cancer and other human diseases. Consequently, a greater understanding of the factors regulating cell cycle progression becomes essential to help enhance to response of patients to these drugs. Here we identified the poorly-studied factor FAM53C as a new regulator of the G1/S transition. We first used data from the Cancer Dependency Map to identify new candidate regulators of the G1/S transition, and validated FAM53C as necessary and sufficient for this cell cycle transition. Genetic approaches showed that FAM53C acts upstream of the RB tumor suppressor. By mass spectrometry, we identified and validated DYRK1A as a kinase normally inhibited by FAM53C. DYRK1A is an upstream negative regulator of Cyclin D, which also negatively regulates RB. Fam53C knockout mice are viable but show defects in growth. Together, these experiments identify FAM53C as a regulator of DYRK1A and of cell cycle progression in G1.
HostingRepository	PRIDE
AnnounceDate	2026-04-23
AnnouncementXML	Submission_2026-04-22_20:30:31.365.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD055829
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Janos Demeter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 17:38:50	ID requested
⏵ 1	2026-04-22 20:30:31	announced

10.6019/PXD055829;

submitter keyword: G1/S transition,Cell cycle

Peter Kent Jackson
contact affiliation	Baxter Laboratory for Stem Cell Biology Department of Microbiology & Immunology Stanford University School of Medicine
contact email	pjackson@stanford.edu
lab head
Janos Demeter
contact affiliation	Stanford University
contact email	jdemeter@stanford.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055829
     1. Label: PRIDE project
     2. Name: THE FAM53C/DYRK1A AXIS REGULATES THE G1/S TRANSITION OF THE CELL CYCLE