PXD055823

PXD055823 is an original dataset announced via ProteomeXchange.

Title	A proteomic approach to identify vaccine candidates based on host cell attachment provides a novel protective Pseudomonas aeruginosa vaccine antigen: cell division protein, FtsZ
Description	Pseudomonas aeruginosa is an opportunistic Gram negative pathogen that causes severe nosocomial infections in susceptible individuals due to the emergence of multi-drug resistant strains. There are no approved vaccines against P. aeruginosa infections nor candidates in active clinical development, highlighting the need for novel candidates and strategies. Using a cell-blot proteomic approach, we reproducibly identified 49 proteins involved in interactions with human lung epithelial cells across four P. aeruginosa strains. Among these were cell division protein FtsZ and outer membrane protein OpmH. Escherichia coli BL21 cells overexpressing recombinant FtsZ or rOpmH showed a 66- and 15-fold increased ability to attach to 16HBE14o- cells, further supporting their involvement in host cell attachment. Both antigens led to proliferation of NK and CD8+ cytotoxic T cells, significant increases in the production of IFN-γ, IL-17A, TNF and IL-4 in immunised mice and elicited strong antigen-specific serological IgG1 and IgG2c responses. Immunisation with FtsZ, significantly reduced bacterial burden in the lungs by 1.9-log CFU and dissemination to spleen (1.8-log CFU). The protective candidate, FtsZ, would not have been identified by traditional approaches relying on either virulence mechanisms or sequence-based predictions, opening new avenues in the development of an anti-P. aeruginosa vaccine.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:02:51.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Irene Jurado Martin
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 08:44:02	ID requested
⏵ 1	2025-05-07 03:02:52	announced

10.1038/s41541-024-00994-x;

Jurado-Mart, í, n I, Tom, á, s-Cort, á, zar J, Hou Y, Sainz-Mej, í, as M, Mysior MM, Sadon, è, s O, Huebner J, Romero-Saavedra F, Simpson JC, Baugh JA, McClean S, Proteomic approach to identify host cell attachment proteins provides protective Pseudomonas aeruginosa vaccine antigen FtsZ. NPJ Vaccines, 9(1):204(2024) [pubmed]

submitter keyword: antigens,Pseudomonas aeruginosa, vaccine

Siobhán McClean
contact affiliation	UCD Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Dublin, Ireland
contact email	siobhan.mcclean@ucd.ie
lab head
Irene Jurado Martin
contact affiliation	University College Dublin
contact email	irene.juradomartin@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055823

PRIDE project URI

• PRIDE
  1. PXD055823
     1. Label: PRIDE project
     2. Name: A proteomic approach to identify vaccine candidates based on host cell attachment provides a novel protective Pseudomonas aeruginosa vaccine antigen: cell division protein, FtsZ