PXD055817

PXD055817 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of rat periodontal tissues in health and periodontal disease
Description	The periodontal ligament tissue plays a crucial role in oral functions by providing structural support to the tooth. Due to the presence of undifferentiated mesenchymal cells, research into its regenerative capacity is ongoing. Pathological conditions can affect these functions and protein composition. The aim of this study was to evaluate through proteomic analysis the protein profile of the periodontal ligament (PDL) tissues of rats submitted to experimental periodontitis induction. The study was approved by the Ethical Committee on Animal Experimentation of the Sao Paulo State University, School of Dentistry at Araraquara, Brazil (protocol number 16/2015) and followed the Animal Research: Reporting of In Vivo Experiments guidelines. A total of eight male adult Holtzman rats, average weight of 300 g, were used and housed in plastic cages properly identified, with a 12-hours lights/dark cycle, under controlled temperature (22-25 °C), receiving water ad libitum and standard laboratory diet (Labina/Purina, Ribeirão Preto, Brazil). The animals were randomly assigned into two groups: control without any intervention (C, n=3) and experimental periodontitis (P, n=5). The animals of the group P were anesthetized with intramuscular injections of 10% ketamine chlorhydrate (0.08 ml/100 g body weight) and 2% xylazine chlorhydrate (0.04 ml/100 g body weight). To induce periodontitis, a cotton thread was placed bilaterally around the upper first molars and maintained with a knot for 20 days. After this period, all animals were euthanized by anesthetic overdose and had their maxillary jaws collected for subsequent analyses.
HostingRepository	PRIDE
AnnounceDate	2025-05-01
AnnouncementXML	Submission_2025-05-01_08:31:27.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Eduarda Lopes
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 06:34:59	ID requested
⏵ 1	2025-05-01 08:31:27	announced

10.1016/J.JPROT.2025.105384;

submitter keyword: LC-MSMS, C5, Periodontal ligament, Lgals3,Proteomics, SerpinB1, Periodontitis

Joni Augusto Cirelli
contact affiliation	Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University - UNESP, Araraquara, São Paulo, Brazil
contact email	joni.cirelli@unesp.br
lab head
Maria Eduarda Lopes
contact affiliation	São Paulo State University (UNESP) - School of dentistry Araraquara
contact email	maria.e.lopes@unesp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055817

PRIDE project URI

• PRIDE
  1. PXD055817
     1. Label: PRIDE project
     2. Name: Proteomic profile of rat periodontal tissues in health and periodontal disease