PXD055776

PXD055776 is an original dataset announced via ProteomeXchange.

Title	Antagonist actions of CMK-1/CaMKI and TAX-6/Calcineurin along the C. elegans thermal avoidance circuit orchestrate nociceptive habituation
Description	In this project, we performed three sets of in vitro kinase assays coupled to phosphoproteomic workflows. (1) tryptic peptides of C. elegans lysate treated with recombinant active CMK-1 (positive2 run) and kinase-dead CMK-1 and 18O labeled ATP (2) immobilized whole cell lysate (native conditions, protein level assay) incubated without ATP (negative control 1), with ATP (negative control 2), and with ATP and with active CMK-1 (positive experiment). After tryptic digestion, peptides were dimethyl-labeled using standard conditions. (3) purified TAX-6 isoform A and C were treated with recombinant active CMK-1 and kinase-dead CMK-1.
HostingRepository	PRIDE
AnnounceDate	2025-04-10
AnnouncementXML	Submission_2025-04-10_00:59:56.650.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joern Dengjel
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 03:44:39	ID requested
⏵ 1	2025-04-10 00:59:57	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, in vitro kinase assay, kinase,phosphorylation

Joern Dengjel
contact affiliation	Department of Biology, University of Fribourg Chemin du Musée 10, 1700 Fribourg, Switzerland T +41 26 300 8631
contact email	joern.dengjel@unifr.ch
lab head
Joern Dengjel
contact affiliation	University of Fribourg
contact email	joern.dengjel@medizin.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD055776

PRIDE project URI

• PRIDE
  1. PXD055776
     1. Label: PRIDE project
     2. Name: Antagonist actions of CMK-1/CaMKI and TAX-6/Calcineurin along the C. elegans thermal avoidance circuit orchestrate nociceptive habituation