PXD055775

PXD055775 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention
Description	Nuclear speckles are membraneless organelles that associate with active transcription sites and participate in post-transcriptional mRNA processing. During the cell cycle, nuclear speckles dissolve following phosphorylation of their protein components. Here, we identify the PP1 family as the phosphatases that counteract kinase-mediated dissolution. PP1 overexpression increases speckle cohesion and leads to retention of polyadenylated RNA within speckles and the nucleus. Using APEX2 proximity labeling combined with RNA-sequencing, we characterized the relationship between the cohesion of nuclear speckles and the recruitment of specific RNAs. We find that many transcripts are preferentially enriched within nuclear speckles compared to the nucleoplasm, particularly chromatin- and nucleus-associated transcripts. While total polyadenylated RNA retention increased with nuclear speckle cohesion, the ratios of most mRNA species to each other were constant, indicating non-selective, or proportional, retention. We then explored whether nuclear speckle cohesion changes in response to environmental perturbations associated with changes in kinase or phosphatase activity. We found that cellular responses to heat shock, oxidative stress, and hypoxia include changes to the cohesion of nuclear speckles and mRNA retention. Our results demonstrate that tuning the material properties of nuclear speckles provides a mechanism for the acute control of mRNA localization.
HostingRepository	PRIDE
AnnounceDate	2024-11-28
AnnouncementXML	Submission_2024-11-28_06:17:56.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katrina Meyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 02:08:05	ID requested
⏵ 1	2024-11-28 06:17:56	announced

Dataset with its publication pending

submitter keyword: nuclear speckles, DYRK3, phosphatase, kinase

Lucas Pelkmans
contact affiliation	Department of Molecular Life Sciences, University of Zurich, Switzerland
contact email	lucas.pelkmans@imls.uzh.ch
lab head
Katrina Meyer
contact affiliation	Max-Planck-Institute for Molecular Genetics
contact email	katrinamey@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055775
     1. Label: PRIDE project
     2. Name: Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention