PXD055767

PXD055767 is an original dataset announced via ProteomeXchange.

Title	Impact of Brilliant blue FCF on Human Gut Microbiota: Structural and Functional Alterations in a Simplified Model System
Description	The human gut microbiota plays a vital role in host health by acting as a barrier against pathogens, boosting the immune system, and metabolizing complex carbon sources into beneficial compounds such as short-chain fatty acids (SCFAs). However, external factors like diet and xenobiotics, including food colorants, can impact this microbial community. Brilliant blue FCF (E-133), a common food dye that is not absorbed or metabolized by the body, thus, leading to significant exposure of the gut microbiota, and its effects on it are not well-documented. This study investigated the impact of brilliant blue on the simplified human gut microbiota model (SIHUMIx) over a seven-day exposure period, followed by a four-day recovery phase. Using continuous culture bioreactors along with metaproteomic and metabolomic analyses, the research uncovered substantial structural and functional changes in the microbiota. Our findings showed alterations in the species abundance such as B. thetaiotaomicron, B. longum, and C. butyricum, reductions in energy metabolism by-products, particularly lactate and butyrate, and disruptions in metabolic pathways involved in the metabolism of xenobiotics and amino acids. The results indicate that brilliant blue exposure compromises microbiota stability and functionality, highlighting the need for further research on its long-term effects and recovery mechanisms. This study underscores the importance of assessing the impacts of food additives on gut microbiota as part of comprehensive health risk evaluations.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:21:27.806.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Castaneda-Monsalve
SpeciesList	scientific name: Escherichia coli (strain K12); NCBI TaxID: 83333; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bifidobacterium longum; NCBI TaxID: 216816; scientific name: Clostridium butyricum DSM 10702; NCBI TaxID: 1316931; scientific name: Blautia producta ATCC 27340 = DSM 2950; NCBI TaxID: 1121114; scientific name: Anaerostipes caccae; NCBI TaxID: 105841; scientific name: Bacteroides thetaiotaomicron; NCBI TaxID: 818; scientific name: Lactobacillus plantarum; NCBI TaxID: 1590; scientific name: Thomasclavelia ramosa; NCBI TaxID: 1547;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-10 05:50:17	ID requested
⏵ 1	2025-05-07 03:21:28	announced

10.1093/ismeco/ycaf050;

Casta, ñ, eda-Monsalve V, Haange SB, Fr, ö, hlich LF, Fu Q, Rolle-Kampczyk U, von Bergen M, Jehmlich N, Food colorant brilliant blue causes persistent functional and structural changes in an in vitro simplified microbiota model system. ISME Commun, 5(1):ycaf050(2025) [pubmed]

submitter keyword: food colorants, metabolomics, brilliant blue, gut microbiota,SIHUMIx, metaproteomics

Nico Jehmlich
contact affiliation	Helmholtz Centre for Environmental Research, UFZ
contact email	nico.jehmlich@ufz.de
lab head
Victor Castaneda-Monsalve
contact affiliation	Helmholtz Centre for Environmental Research (UFZ)
contact email	victor.castaneda@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055767
     1. Label: PRIDE project
     2. Name: Impact of Brilliant blue FCF on Human Gut Microbiota: Structural and Functional Alterations in a Simplified Model System