PXD055727

PXD055727 is an original dataset announced via ProteomeXchange.

Title	LipoID: a Photochemical Proteomic Method to in situ Profile Lipid Droplet Interactions and Identify Regulating Factors
Description	In surveillance of cellular energy homeostasis, lipid droplets (LDs) dynamically interact with other organelles, such as mitochondria, to tightly engage in metabolic regulations. The transient nature of LDs’ interactions, however, poses technical challenges to snapshot the enriched information by classical fractionation methods. Herein, we develop a small molecule based photocatalytic protein proximity labeling method (namely LipoID) to in situ label, capture and profile LDs’ interacting proteome in live cells. To this end, a set of LD-targeting probes are designed to undergo photocatalytic protein modifications in close proximity to LDs by nucleophilic substrates. Photochemical mechanistic studies confirm predominant modification on histidine residues via type I radical pathway. We show the LipoID method identifies inter-organellar interactions, particularly with mitochondria, besides reliable capture of validated LDs’ biomarkers (e.g. Plins). We also exemplify LipoID coupled with comparative proteomic analysis discovers key mitochondrial channel VDAC3 to regulate the inter-organellar distance between LDs and mitochondria. Together, the small molecule based LipoID method reported herein allows us to in situ and de novo study LD interactions without genetic-encoding a functional tag to a known biomarker.
HostingRepository	iProX
AnnounceDate	2024-09-07
AnnouncementXML	Submission_2024-09-09_01:11:50.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ci Wu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-09 01:11:23	ID requested
⏵ 1	2024-09-09 01:11:50	announced

Dataset with its publication pending

submitter keyword: lipid droplets

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Ci Wu
contact affiliation	Chinese Academy of Sciences
contact email	wuci1111@126.com
dataset submitter

iProX dataset URI