PXD055708

PXD055708 is an original dataset announced via ProteomeXchange.

Title	Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells
Description	Proximity labeling (PL) characterizes protein interactome in living cells. However, exogenous H2O2 required for engineered peroxidase, like APEX2, is cytotoxic, while biotin ligases-based PL have poor temporal resolution. Here, we showed that SOPP3, an engineered photosensitizer, could trigger APEX2 mediated PL within seconds under blue light illumination, without exogenous H2O2 or cytotoxicity. This new PL technology paves an avenue to characterize molecular dynamics of key biomedical events with high spatial-temporal resolution, efficiency, and flexible applicability.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:54:37.640.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-08 21:06:27	ID requested
⏵ 1	2025-05-07 02:54:38	announced

10.1038/s41422-024-01061-9;

Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang W, Pan W, Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells. Cell Res, 35(2):149-152(2025) [pubmed]

submitter keyword: proximity labeling, APEX2, SOPP3, protein-protein interaction, photosensitizer

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Qian Liu
contact affiliation	Shanghai Institute of Materia Medica
contact email	liuqian_scu@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055708

PRIDE project URI

• PRIDE
  1. PXD055708
     1. Label: PRIDE project
     2. Name: Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells