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PXD055708

PXD055708 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhotoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells
DescriptionProximity labeling (PL) characterizes protein interactome in living cells. However, exogenous H2O2 required for engineered peroxidase, like APEX2, is cytotoxic, while biotin ligases-based PL have poor temporal resolution. Here, we showed that SOPP3, an engineered photosensitizer, could trigger APEX2 mediated PL within seconds under blue light illumination, without exogenous H2O2 or cytotoxicity. This new PL technology paves an avenue to characterize molecular dynamics of key biomedical events with high spatial-temporal resolution, efficiency, and flexible applicability.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_02:54:37.640.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQian Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-08 21:06:27ID requested
12025-05-07 02:54:38announced
Publication List
10.1038/s41422-024-01061-9;
Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang W, Pan W, Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells. Cell Res, 35(2):149-152(2025) [pubmed]
Keyword List
submitter keyword: proximity labeling, APEX2, SOPP3, protein-protein interaction, photosensitizer
Contact List
Hu Zhou
contact affiliationShanghai Institute of Materia Medica
contact emailzhouhu@simm.ac.cn
lab head
Qian Liu
contact affiliationShanghai Institute of Materia Medica
contact emailliuqian_scu@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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