PXD055708 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells |
Description | Proximity labeling (PL) characterizes protein interactome in living cells. However, exogenous H2O2 required for engineered peroxidase, like APEX2, is cytotoxic, while biotin ligases-based PL have poor temporal resolution. Here, we showed that SOPP3, an engineered photosensitizer, could trigger APEX2 mediated PL within seconds under blue light illumination, without exogenous H2O2 or cytotoxicity. This new PL technology paves an avenue to characterize molecular dynamics of key biomedical events with high spatial-temporal resolution, efficiency, and flexible applicability. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_02:54:37.640.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qian Liu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-08 21:06:27 | ID requested | |
⏵ 1 | 2025-05-07 02:54:38 | announced | |
Publication List
10.1038/s41422-024-01061-9; |
Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang W, Pan W, Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells. Cell Res, 35(2):149-152(2025) [pubmed] |
Keyword List
submitter keyword: proximity labeling, APEX2, SOPP3, protein-protein interaction, photosensitizer |
Contact List
Hu Zhou |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | zhouhu@simm.ac.cn |
lab head | |
Qian Liu |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | liuqian_scu@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055708 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055708
- Label: PRIDE project
- Name: Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells