PXD055651

PXD055651 is an original dataset announced via ProteomeXchange.

Title	Deletion of ASPP1 in myofibroblasts alleviates myocardial fibrosis by reducing p53 degradation
Description	In the healing process of myocardial infarction, cardiac fibroblasts are activated to produce collagen, leading to adverse remodeling and heart failure. Our previous study showed that ASPP1 promotes cardiomyocyte apoptosis by enhancing nuclear trafficking of p53. We thus explored the influence of ASPP1 on myocardial fibrosis and the underlying mechanisms. Here, we observed ASPP1 was increased after 4 weeks of MI. Both global and myofibroblast knockout of ASPP1 in mice mitigated cardiac dysfunction and fibrosis after MI. Strikingly, ASPP1 produced opposite influence on p53 level and cell fate in cardiac fibroblasts and cardiomyocytes. Knockdown of ASPP1 increased p53 level and inhibited the activity of cardiac fibroblasts. ASPP1 accumulated in the cytoplasm of fibroblasts while the level of p53 was reduced following TGF-1 stimulation; however, inhibition of ASPP1 increased p53 level and promoted p53 nuclear translocation. Mechanistically, ASPP1 directly bound to deubiquitinase OTUB1, thereby promoting the ubiquitination and degradation of p53, attenuating myofibroblast activity and cardiac fibrosis, and improving heart function after MI.
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_19:57:22.440.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shangxuan Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-07 03:40:28	ID requested
⏵ 1	2025-01-17 19:57:23	announced

Li S, Yang M, Zhao Y, Zhai Y, Sun C, Guo Y, Zhang X, Zhang L, Tian T, Yang Y, Pei Y, Li J, Li C, Xuan L, Li X, Zhao D, Yang H, Zhang Y, Yang B, Zhang Z, Pan Z, Lu Y, Deletion of ASPP1 in myofibroblasts alleviates myocardial fibrosis by reducing p53 degradation. Nat Commun, 15(1):8425(2024) [pubmed]

10.1038/s41467-024-52739-y;

submitter keyword: LC-MS,Primary mouse cardiac fibroblasts, ASPP1

Shangxuan Li
contact affiliation	State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), Department of Pharmacology (State Key Labratoray -Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150081, China.
contact email	1191398969@qq.com
lab head
Shangxuan Li
contact affiliation	State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), Department of Pharmacology (State Key Labratoray -Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University.
contact email	1191398969@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD055651

PRIDE project URI

• PRIDE
  1. PXD055651
     1. Label: PRIDE project
     2. Name: Deletion of ASPP1 in myofibroblasts alleviates myocardial fibrosis by reducing p53 degradation