PXD055641

PXD055641 is an original dataset announced via ProteomeXchange.

Title	EBI2/oxysterol signalling modulates the expression of VE-cadherin and attachment of multiple sclerosis CD4+ T cells to an in vitro blood-brain barrier model
Description	Changes in the function of the blood-brain barrier (BBB) is one of the hallmarks of multiple sclerosis (MS) and is observed at very early stages of the disease. Several disease-modifying therapies for MS regulate tight junction and adherence proteins in the BBB thus limiting the entry of peripheral immune cells into the central nervous system (CNS). The Epstein-Barr virus-induced gene 2 (EBI2) was shown to drive immune cell migration towards high concentration of its endogenous ligand, oxysterol 7α,25OHC, which increases during inflammation in the CNS. Here, the data showed upregulated transcripts of EBI2 and CH25H, the first enzyme in 7α,25OHC synthesis pathway, in MS plaques. In vitro, oxysterols and cerebrospinal fluid (CSF) from MS patients downregulated HSD3B7, the 7α,25OHC degrading enzyme, and VE-cadherin levels in the tri-cell human BBB model. Importantly, EBI2 signalling mediated the attachment of MS patient-derived CD4+ T cells to the BBB spheroids. The data raises the possibility that elevated oxysterol levels in an inflamed brain might trigger a reduction in VE-cadherin expression in endothelial cells, potentially easing the CNS infiltration of EBI2-expressing immune cells. This process can be modulated through the use of EBI2 ligands, suggesting a potential pathway for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_16:52:47.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inez Mruk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-06 12:51:53	ID requested
⏵ 1	2025-07-20 16:52:48	announced

10.1016/j.bbih.2025.101045;

Caratis F, Mruk I, Konieczna-Wolska K, Rojek B, Ha, ł, as M, Czaplewska P, Karaszewski B, Furihata T, Rutkowska A, T cell attachment to a human tri-cell spheroid blood-brain barrier model. Brain Behav Immun Health, 47():101045(2025) [pubmed]

submitter keyword: Multiple sclerosis, human brain

Paulina Czaplewska
contact affiliation	Intercollegiate Faculty of Biotechnology, Laboratory of Mass Spectrometry, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
Inez Mruk
contact affiliation	Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk
contact email	inez.mruk@phdstud.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055641

PRIDE project URI

• PRIDE
  1. PXD055641
     1. Label: PRIDE project
     2. Name: EBI2/oxysterol signalling modulates the expression of VE-cadherin and attachment of multiple sclerosis CD4+ T cells to an in vitro blood-brain barrier model