PXD055633

PXD055633 is an original dataset announced via ProteomeXchange.

Title	Non-classical neutrophil extracellular traps induced by PAR2-signaling proteases
Description	Neutrophil extracellular traps (NETs) are associated with diseases linked to aberrant coagulation. The blood clotting cascade involves a series of proteases, some of which induce NETs formation via a yet unknown mechanism. We hypothesized that this formation involves signaling via a factor Xa (FXa) activation of the protease activated receptor 2 (PAR2). Our findings revealed that NETs can be triggered in vitro by enzymatically active proteases and PAR2 agonists. Intravital microscopy of the liver vasculature revealed that both FXa infusion and activation of endogenous FX promoted NET formation, effects that were prevented by the FXa inhibitor, apixaban. Unlike classical NETs, these protease-induced NETs lacked bactericidal activity and their proteomic signature indicates their role in inflammatory disorders, including autoimmune diseases and carcinogenesis. Our findings suggest a novel mechanism of NET formation under aseptic conditions, potentially contributing to a self-amplifying cycle of clotting and NETs formation. This mechanism may underlie the pathogenesis of disseminated intravascular coagulation and other aseptic conditions.
HostingRepository	PRIDE
AnnounceDate	2025-02-24
AnnouncementXML	Submission_2025-02-24_05:07:28.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Kowalczyk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-06 08:13:38	ID requested
⏵ 1	2025-02-24 05:07:28	announced

10.1038/S41419-025-07428-Z;

submitter keyword: NETs, AC264613,Neutrophil extracellular traps, PAR2-signaling

Joanna Koziel
contact affiliation	Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
contact email	joanna.koziel@uj.edu.pl
lab head
Dominik Kowalczyk
contact affiliation	Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
contact email	dominik.kowalczyk@doctoral.uj.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD055633

PRIDE project URI

• PRIDE
  1. PXD055633
     1. Label: PRIDE project
     2. Name: Non-classical neutrophil extracellular traps induced by PAR2-signaling proteases