PXD055627 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Two redox-responsive LysR-type transcription factors in the phytopathogen Agrobacterium tumefaciens |
Description | Bacteria are constantly exposed to fluctuations in reactive oxygen species (ROS). In particular, pathogenic bacteria encounter elevated ROS-levels during host infection. In our present study we investigated the intricate regulatory interplay of the LysR-type transcriptional regulators LsrB and OxyR in Agrobacterium tumefaciens. OxyR is a widespread thiol-based redox sensor in bacteria, while LsrB is unique to Rhizobiales. Using phenotypic assays and RNA-sequencing under oxidative stress, we unveiled a central role for both regulators in co-regulating key antioxidant pathways, including katG encoding a bifunctional catalase/peroxidase. Furthermore, we provide novel insights into the mechanism of LsrB activation, suggesting that LsrB also functions as a thiol-based redox sensor. In summary, our findings illuminate the LsrB-OxyR system as a critical component of A. tumefaciens' defense against oxidative stress |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-28 |
AnnouncementXML | Submission_2025-04-27_23:04:29.596.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sina Schäkermann |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | methylthiolated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-06 04:59:04 | ID requested | |
⏵ 1 | 2025-04-27 23:04:30 | announced | |
Publication List
Keyword List
submitter keyword: LsrB,LTTR (LysR-type transcriptional regulator), stress response, ROS, oxidative stress, OxyR, Agrobacterium, thiol-based redox switch |
Contact List
Julia E. Bandow |
contact affiliation | Ruhr University Bochum, Faculty of Biology and Biotechnology, Applied Microbiology, Universitätsstraße 150, 44801 Bochum, Germany Center for System-based Antibiotic Research, Ruhr University Bochum, Germany |
contact email | julia.bandow@rub.de |
lab head | |
Sina Schäkermann |
contact affiliation | Ruhr-Universität Bochum |
contact email | Sina.Langklotz@rub.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055627
- Label: PRIDE project
- Name: Two redox-responsive LysR-type transcription factors in the phytopathogen Agrobacterium tumefaciens