PXD055627

PXD055627 is an original dataset announced via ProteomeXchange.

Title	Two redox-responsive LysR-type transcription factors in the phytopathogen Agrobacterium tumefaciens
Description	Bacteria are constantly exposed to fluctuations in reactive oxygen species (ROS). In particular, pathogenic bacteria encounter elevated ROS-levels during host infection. In our present study we investigated the intricate regulatory interplay of the LysR-type transcriptional regulators LsrB and OxyR in Agrobacterium tumefaciens. OxyR is a widespread thiol-based redox sensor in bacteria, while LsrB is unique to Rhizobiales. Using phenotypic assays and RNA-sequencing under oxidative stress, we unveiled a central role for both regulators in co-regulating key antioxidant pathways, including katG encoding a bifunctional catalase/peroxidase. Furthermore, we provide novel insights into the mechanism of LsrB activation, suggesting that LsrB also functions as a thiol-based redox sensor. In summary, our findings illuminate the LsrB-OxyR system as a critical component of A. tumefaciens' defense against oxidative stress
HostingRepository	PRIDE
AnnounceDate	2025-04-28
AnnouncementXML	Submission_2025-04-27_23:04:29.596.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sina Schäkermann
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	methylthiolated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-06 04:59:04	ID requested
⏵ 1	2025-04-27 23:04:30	announced

10.1093/NAR/GKAF267;

submitter keyword: LsrB,LTTR (LysR-type transcriptional regulator), stress response, ROS, oxidative stress, OxyR, Agrobacterium, thiol-based redox switch

Julia E. Bandow
contact affiliation	Ruhr University Bochum, Faculty of Biology and Biotechnology, Applied Microbiology, Universitätsstraße 150, 44801 Bochum, Germany Center for System-based Antibiotic Research, Ruhr University Bochum, Germany
contact email	julia.bandow@rub.de
lab head
Sina Schäkermann
contact affiliation	Ruhr-Universität Bochum
contact email	Sina.Langklotz@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD055627

PRIDE project URI

• PRIDE
  1. PXD055627
     1. Label: PRIDE project
     2. Name: Two redox-responsive LysR-type transcription factors in the phytopathogen Agrobacterium tumefaciens