PXD055625

PXD055625 is an original dataset announced via ProteomeXchange.

Title	eIF2A regulates cell migration without overtly affecting translation
Description	The RNA-binding protein eIF2A has been implicated in a variety of cellular processes including tumorigenesis. This role has been attributed to its function as alternative translation initiation factor. However, the mechanisms by which eIF2A regulates translation and its contribution to oncogenic transformation are unclear. Here, we shed light on these aspects using a melanoma cell model consisting of the non-tumoral melanocytic cell line MelST and its metastatic counterpart obtained by RasV12 overexpression (MelSTR). Depletion of eIF2A from MelST and MelSTR cells revealed acquired dependencies upon Ras transformation for migration. Surprisingly, analysis of the transcriptome (RNA-Seq) and translatome (ribosome profiling) upon eIF2A depletion showed minor to no changes in translation. RIP-Seq and RT-qPCR furthermore indicate that eIF2A binds mRNA targets in a translation-independent manner. Interestingly, protein interactome analyses point towards a function of eIF2A in cytoskeletal remodeling and indeed we can show that eIF2A localizes to the centrosome and affects its composition and orientation, linking eIF2A with migration. In addition, eIF2A promotes migration in a manner that depends on its RNA-binding activity. Together, these results indicate that eIF2A does not function as a translation factor in melanoma cells, but through a novel function which is based on its RNA-binding activity and its connections to the centrosome.
HostingRepository	PRIDE
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-05_02:52:19.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Neus Mestre Farràs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-06 04:53:28	ID requested
⏵ 1	2025-08-05 02:52:20	announced

10.1126/sciadv.adu5668;

Jungfleisch J, Mestre-Farr, à, s N, G, ó, mez-Riera R, Pourcelot O, Bertrand E, Halidi N, Gebauer F, eIF2A regulates cell migration in a translation-independent manner. Sci Adv, 11(31):eadu5668(2025) [pubmed]

submitter keyword: Melanoma, immunoprecipitation Mass spectrometry, eIF2A

Fátima Gebauer
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona (BIST), Barcelona, Spain. Universitat Pompeu Fabra (UPF), Barcelona, Spain.
contact email	fatima.gebauer@crg.eu
lab head
Neus Mestre Farràs
contact affiliation	Centre Regulació Genòmica
contact email	neus.mestre@crg.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD055625

PRIDE project URI

• PRIDE
  1. PXD055625
     1. Label: PRIDE project
     2. Name: eIF2A regulates cell migration without overtly affecting translation