PXD055614

PXD055614 is an original dataset announced via ProteomeXchange.

Title	Proteins of sheep abomasum that interact with Hc-SPI-I8
Description	Freshly collected abomasum tissue of the naturally infected sheep was ground, filtered, and centrifuged at 3000 g at 4 °C for cell collection. The collected cells were lysed in NP-40 lysis buffer (Thermo Fisher Scientific) for 0.5 h with shaking, then centrifuged at 12000 g at 4 °C for supernatant collection. Coding sequence of Hc-spi-i8 (HCON_00067680) was amplified and inserted into pcDNA3.1(+) vector to produce FLAG-tagged Hc-SPI-I8 in HEK 293T cells. The recombinant Hc-SPI-I8-FLAG protein was isolated from lysed cells using anti-FLAG-conjugated magnetic beads (Bimake, Shanghai, China), and used to isolate and concentrate interactive proteins from the ovine tissue lysis. The isolated proteins were boiled, separated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), from which proteins within a range of molecular weights from 30 to 45 kDa were subjected to digestion with trypsin (Promega), desalination with a sep-Pak C18 (Waters) and LC-MS/MS analysis with a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific). Peptides were identified and annotated using Proteome Discoverer v.2.1.
HostingRepository	iProX
AnnounceDate	2024-09-06
AnnouncementXML	Submission_2025-09-08_00:13:58.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fei Wu
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-05 23:44:55	ID requested
1	2024-09-05 23:45:36	announced
⏵ 2	2025-09-08 00:13:59	announced	2025-09-08: Add info.

Wu F, Chen Y, Chen X, Tong D, Zhou J, Du Z, Yao C, Yang Y, Du A, Ma G, B signalling by hijacking MKRN1-mediated polyubiquitination of RACK1. Commun Biol, 8(1):356(2025) [pubmed]

submitter keyword: Haemonchus contortus, IP-MS, host-parasite interplay, protein-protein interaction

Guangxu Ma
contact affiliation	Zhejiang University
contact email	gxma1@zju.edu.cn
lab head
Fei Wu
contact affiliation	Zhejiang University
contact email	wufei0214@zju.edu.cn
dataset submitter

iProX dataset URI