PXD055614
PXD055614 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteins of sheep abomasum that interact with Hc-SPI-I8 |
Description | Freshly collected abomasum tissue of the naturally infected sheep was ground, filtered, and centrifuged at 3000 g at 4 °C for cell collection. The collected cells were lysed in NP-40 lysis buffer (Thermo Fisher Scientific) for 0.5 h with shaking, then centrifuged at 12000 g at 4 °C for supernatant collection. Coding sequence of Hc-spi-i8 (HCON_00067680) was amplified and inserted into pcDNA3.1(+) vector to produce FLAG-tagged Hc-SPI-I8 in HEK 293T cells. The recombinant Hc-SPI-I8-FLAG protein was isolated from lysed cells using anti-FLAG-conjugated magnetic beads (Bimake, Shanghai, China), and used to isolate and concentrate interactive proteins from the ovine tissue lysis. The isolated proteins were boiled, separated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), from which proteins within a range of molecular weights from 30 to 45 kDa were subjected to digestion with trypsin (Promega), desalination with a sep-Pak C18 (Waters) and LC-MS/MS analysis with a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific). Peptides were identified and annotated using Proteome Discoverer v.2.1. |
HostingRepository | iProX |
AnnounceDate | 2024-09-06 |
AnnouncementXML | Submission_2024-09-05_23:45:35.965.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fei Wu |
SpeciesList | scientific name: Ovis aries; NCBI TaxID: 9940; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-09-05 23:44:55 | ID requested | |
⏵ 1 | 2024-09-05 23:45:36 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Haemonchus contortus, IP-MS, host-parasite interplay, protein-protein interaction |
Contact List
Guangxu Ma | |
---|---|
contact affiliation | Zhejiang University |
contact email | gxma1@zju.edu.cn |
lab head | |
Fei Wu | |
contact affiliation | Zhejiang University |
contact email | wufei0214@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |