PXD055601

PXD055601 is an original dataset announced via ProteomeXchange.

Title	An Analysis of Toxoplasma gondii Extracellular Vesicles.
Description	Toxoplasma gondii, the causative agent of toxoplasmosis, infects roughly one-third of the global population, highlighting its remarkable adaptability and pathogenicity across a wide range of warm-blooded animals. A key aspect of T. gondii’s ability to interact with its hosts involves the secretion of extracellular vesicles (EVs), which play a crucial role in mediating host-parasite interactions. These EVs carry a variety of molecular cargoes, including proteins, lipids, and RNA, which can alter host cellular responses and promote parasite survival. This project focuses on understanding how T. gondii adapts its EVs to the host environment. To explore this, we analyzed the EV cargo of T. gondii cultured in four different host cell lines representing distinct species. Each cell type provides a unique environment that mimics the conditions encountered by T. gondii during infection. By comparing the EV compositions across these cell lines, we aim to identify the diverse adaptive strategies that T. gondii employs through its EVs. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we characterized the EVs and conducted a comprehensive quantitative analysis of their proteomic cargo. Our findings revealed notable differences in the abundance and composition of EVs produced by tachyzoites cultivated in distinct host environments, underscoring the significant influence of host cell type on the makeup of T. gondii EVs.
HostingRepository	PRIDE
AnnounceDate	2025-03-19
AnnouncementXML	Submission_2025-03-19_02:07:15.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Teresa Cruz Bustos
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-05 15:00:52	ID requested
⏵ 1	2025-03-19 02:07:15	announced

10.3389/FCIMB.2025.1565684;

submitter keyword: toxoplasma gondii

exovesicles

EVs

Anja Joachim
contact affiliation	lab_head_affiliation institute parasitology, University of veterinary medicine vienna, Veterinärplatz 1, 1210 vienna, austria
contact email	Anja.Joachim@vetmeduni.ac.at
lab head
Teresa Cruz Bustos
contact affiliation	Unit of Parasitology, center of pathobiology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna,
contact email	teresa.cruz-bustos@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD055601

PRIDE project URI

• PRIDE
  1. PXD055601
     1. Label: PRIDE project
     2. Name: An Analysis of Toxoplasma gondii Extracellular Vesicles.