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PXD055583

PXD055583 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHormone-mediated disassembly and inactivation of a plant E3 ubiquitin ligase complex
DescriptionPhytohormone abscisic acid (ABA) regulates key aspects of plant development such as seed germination, as well as responses to important environmental stresses, including drought, salinity and cold temperatures. The ABA signaling pathway is tightly controlled by the ubiquitin proteasome system. CULLIN4-RING E3 ubiquitin ligases use the substrate receptor module COP10-DDB1-DET1-DDA1 (CDDD) to target PYL8, one of the ABA receptors of the PYR/PYL/RCAR (pyrabactin resistance/pyrabactin resistance-like/regulatory components of ABA) family. Therefore, CRL4-CDDD complexes are negative regulators of ABA-mediated stress responses. Conversely, ABA treatments attenuate PYL8 receptor degradation, although the precise molecular details of this mechanism remained unknown. Here, we show that ABA promotes the disruption of CRL4-CDDD complexes, leading to PYL8 stabilization. ABA-mediated CRL4-CDDD dissociation likely involves altered association between DDA1-containing complexes and the CSN, a master regulator of the assembly of cullin-based E3 ligases, including CRL4-CDDD. Indeed, treatments with CSN5i-3, an inhibitor of the CSN activity, suppressed the ABA effect on CRL4-CDDD assembly. Altogether, our findings indicate that ABA stabilizes PYL8 by altering the dynamics of the CRL4-CDDD-CSN complex association, unveiling a regulatory mechanism by which a plant hormone inhibits an E3 ubiquitin ligase to protect its own receptors from degradation.
HostingRepositoryPRIDE
AnnounceDate2024-09-11
AnnouncementXMLSubmission_2024-09-11_03:06:00.172.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD055583
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlberto Paradela
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-05 05:36:26ID requested
12024-09-11 03:06:00announced
Publication List
10.6019/PXD055583;
Keyword List
submitter keyword: ABA signaling, Arabidopsis, CSN, DDA1, Plant Proteostasis, CSN5 inhibitor, PYR/PYL/RCAR, ABA receptor, PYL8, CRL4 E3 ligases
Contact List
Vicente Rubio
contact affiliationDepartment of Plant Molecular Genetics National Center for Biotechnology Spanish Research Council Darwin 3 28049 Madrid Spain
contact emailvrubio@cnb.csic.es
lab head
Alberto Paradela
contact affiliationProteomics Lab CNB-CSIC
contact emailalberto.paradela@cnb.csic.es
dataset submitter
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