PXD055583

PXD055583 is an original dataset announced via ProteomeXchange.

Title	Hormone-mediated disassembly and inactivation of a plant E3 ubiquitin ligase complex
Description	Phytohormone abscisic acid (ABA) regulates key aspects of plant development such as seed germination, as well as responses to important environmental stresses, including drought, salinity and cold temperatures. The ABA signaling pathway is tightly controlled by the ubiquitin proteasome system. CULLIN4-RING E3 ubiquitin ligases use the substrate receptor module COP10-DDB1-DET1-DDA1 (CDDD) to target PYL8, one of the ABA receptors of the PYR/PYL/RCAR (pyrabactin resistance/pyrabactin resistance-like/regulatory components of ABA) family. Therefore, CRL4-CDDD complexes are negative regulators of ABA-mediated stress responses. Conversely, ABA treatments attenuate PYL8 receptor degradation, although the precise molecular details of this mechanism remained unknown. Here, we show that ABA promotes the disruption of CRL4-CDDD complexes, leading to PYL8 stabilization. ABA-mediated CRL4-CDDD dissociation likely involves altered association between DDA1-containing complexes and the CSN, a master regulator of the assembly of cullin-based E3 ligases, including CRL4-CDDD. Indeed, treatments with CSN5i-3, an inhibitor of the CSN activity, suppressed the ABA effect on CRL4-CDDD assembly. Altogether, our findings indicate that ABA stabilizes PYL8 by altering the dynamics of the CRL4-CDDD-CSN complex association, unveiling a regulatory mechanism by which a plant hormone inhibits an E3 ubiquitin ligase to protect its own receptors from degradation.
HostingRepository	PRIDE
AnnounceDate	2024-09-11
AnnouncementXML	Submission_2024-09-11_03:06:00.172.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055583
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alberto Paradela
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-05 05:36:26	ID requested
⏵ 1	2024-09-11 03:06:00	announced

10.6019/PXD055583;

submitter keyword: ABA signaling, Arabidopsis, CSN, DDA1, Plant Proteostasis, CSN5 inhibitor, PYR/PYL/RCAR, ABA receptor, PYL8, CRL4 E3 ligases

Vicente Rubio
contact affiliation	Department of Plant Molecular Genetics National Center for Biotechnology Spanish Research Council Darwin 3 28049 Madrid Spain
contact email	vrubio@cnb.csic.es
lab head
Alberto Paradela
contact affiliation	Proteomics Lab CNB-CSIC
contact email	alberto.paradela@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055583
     1. Label: PRIDE project
     2. Name: Hormone-mediated disassembly and inactivation of a plant E3 ubiquitin ligase complex