PXD055562

PXD055562 is an original dataset announced via ProteomeXchange.

Title	Serum proteomic analysis of circulating IgA1-containing immune complexes in patients with IgA nephropathy
Description	IgA nephropathy (IgAN) is an autoimmune disease that involves galactose-deficient IgA1 (Gal-deficient IgA1) recognized by autoantibodies to form circulating immune complexes (IgA1-IC). Some of these complexes deposit in the glomeruli and induce kidney injury. Gal-deficient IgA1 alone is not sufficient to induce kidney damage. Additional proteins are required to form nephritogenic complexes. In this study, we isolated different molecular forms of serum IgA1, including IgA1-IC, monomeric (mIgA1), and polymeric (pIgA1) by SEC from patients with IgAN and healthy control donors. These three chromatographic fractions of molecular forms of IgA1 were analyzed by LC-MS. An analysis of the proteomes of the IgA1 molecular forms yielded several serum proteins enriched in the IgA1-IC. These proteins were also enriched in patients with IgAN when compared to healthy controls. The ability to characterize the proteome of IgA1-IC provides critical information relevant to IgAN pathogenesis and the nephritogenic characteristics of the ICs.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-03_02:13:01.575.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055562
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mary Cunningham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-04 13:26:48	ID requested
⏵ 1	2025-11-03 02:13:03	announced

10.1016/j.ekir.2025.07.005;

10.6019/PXD055562;

submitter keyword: proteomics, LC-MS, complement, orbitrap, raw, immune complexes,serum, IgA nephropathy, glomerular disease

Matthew B. Renfrow
contact affiliation	Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham
contact email	renfrow@uab.edu
lab head
Mary Cunningham
contact affiliation	University of Alabama at Birmingham
contact email	bunten@uab.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055562
     1. Label: PRIDE project
     2. Name: Serum proteomic analysis of circulating IgA1-containing immune complexes in patients with IgA nephropathy