PXD055556

PXD055556 is an original dataset announced via ProteomeXchange.

Title	A Split-SUMO-ID proteomics approach to identify the Rad52 and SUMO-dependent interactome of Schizosaccharomyces pombe
Description	A PTM known as sumoylation, manifested by an attachment of a SUMO molecule (small ubiquition-like modifier) to target proteins, may lead to changes in the tagged protein’s function, localization or stability. This PTM is present in all eukaryotes. In fission yeast, Rad52 is a DNA repair mediator protein, which enables stalled replicatory fork restart via a homologous recombination mechanism. Fork stalling was postulated to rely on the so-called SUMOylation waves of certain replisome components. Within the project we aimed to identify the protein content at the Rad52-dependent repair sites, which undergo a SUMO wave event. For this purpose, a peculiar proximity labelling approach was uptaken, which is based on fusing two interaction partners to distinct domains (N and C terminal) of a TurboID biotin ligase. Only upon partners’ contact, the ligase is reconstituted and active to label nearby proteins with a biotin molecule, hence providing elusive means of specifically capturing interaction-dependent interactomes.
HostingRepository	PRIDE
AnnounceDate	2025-10-31
AnnouncementXML	Submission_2025-10-31_07:28:07.199.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055556
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NEWT:4896;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-04 10:29:18	ID requested
⏵ 1	2025-10-31 07:28:07	announced

10.6019/PXD055556;

10.1038/S41467-025-65862-1;

submitter keyword: None

Michal Tracz
contact affiliation	Laboratory of Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD055556

PRIDE project URI

• PRIDE
  1. PXD055556
     1. Label: PRIDE project
     2. Name: A Split-SUMO-ID proteomics approach to identify the Rad52 and SUMO-dependent interactome of Schizosaccharomyces pombe