PXD055523

PXD055523 is an original dataset announced via ProteomeXchange.

Title	A microfluidic co-culture platform to generate synaptically separated networks that uncover the role of astrocytes in the initiation and progression of neuron pathology
Description	Communication between astrocytes and neurons plays a pivotal role in the development, maintenance and dysfunction of cellular networks within the central nervous system. Further understanding the contribution of astrocytes to the initiation and progression of neuropathology has, however, been hindered by the complexity of cell networks in traditional in vivo and ex vivo systems. We have designed and constructed a novel three-compartment microfluidic cell culture device that enables us to overcome these limitations and uncouple the roles of astrocytes and neurons in the transfer of pathology through complex cell networks. Our microfluidic device integrates a novel maze-like structure that prevents synaptic connectivity between two fluidically isolated neuron populations, while allowing astrocyte infiltration and growth throughout. We use primary neuron/astrocyte co-cultures, proteomic analysis and immunocytochemistry to validate the application of this device. Using this device, we identify and describe a novel calcium-dependent role for astrocytes in the transfer of excitotoxic pathology between the segregated neuron populations.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:22:34.918.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Wilson
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-03 17:38:15	ID requested
⏵ 1	2026-06-07 16:22:35	announced

Yap YC, Musgrove RE, Breadmore MC, Guijt RM, Wilson R, Wertheimer G, King AE, Dickson TC, Microfluidic co-culture system for synaptically segregated neural networks to explore astrocyte-driven neural pathology. Microsyst Nanoeng, 12(1):(2026) [pubmed]

10.1038/s41378-026-01187-3;

submitter keyword: astrocytes, proteomics, Microfluidics,Neuropathology

Dr Richard Wilson
contact affiliation	Proteomics facility, Central Science Laboratory, University of Tasmania
contact email	richard.wilson@utas.edu.au
lab head
Richard Wilson
contact affiliation	University of Tasmania
contact email	richard.wilson@utas.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD055523

PRIDE project URI

• PRIDE
  1. PXD055523
     1. Label: PRIDE project
     2. Name: A microfluidic co-culture platform to generate synaptically separated networks that uncover the role of astrocytes in the initiation and progression of neuron pathology